Department of Food Engineering, Faculty of Engineering, Pamukkale University, Kinikli, Denizli, Turkey,
Cytotechnology. 2014 Jan;66(1):159-68. doi: 10.1007/s10616-013-9549-3. Epub 2013 May 29.
Human dipeptidylpeptidase IV (hDPPIV) is an enzyme that is in hydrolase class and has various roles in different parts of human body. Its deficiency may cause some disorders in the gastrointestinal, neurologic, endocrinological and immunological systems of humans. In the present study, hDPPIV enzyme was expressed on Spodoptera frugiperda (Sf9) cell lines as a host cell, and the expression of hDPPIV was obtained by a baculoviral expression system. The enzyme production, optimum multiplicity of infection, optimum transfection time, infected and uninfected cell size and cell behavior during transfection were also determined. For maximum hDPPIV (269 mU mL(-1)) enzyme, optimum multiplicity of infection (MOI) and time were 0.1 and 72 h, respectively. The size of infected cells increased significantly (P < 0.001) after 24 h post infection. The results indicated that Sf9 cell line was applicable to the large scale for hDPPIV expression by using optimized parameters (infection time and MOI) because of its high productivity (4.03 mU m L(-1) h(-1)).
人二肽基肽酶 IV（hDPPIV）是一种属于水解酶类的酶，在人体的不同部位具有多种作用。其缺乏可能导致胃肠道、神经、内分泌和免疫系统的一些紊乱。在本研究中，hDPPIV 酶在 Sf9 细胞系上作为宿主细胞表达，并用杆状病毒表达系统获得 hDPPIV 的表达。还确定了酶的生产、最佳感染复数、最佳转染时间、感染和未感染细胞的大小以及转染过程中的细胞行为。为了获得最大的 hDPPIV（269 mU mL(-1)）酶，最佳的感染复数（MOI）和时间分别为 0.1 和 72 h。感染后 24 h，感染细胞的大小显著增加（P < 0.001）。结果表明，Sf9 细胞系适用于通过使用优化的参数（感染时间和 MOI）进行 hDPPIV 的大规模表达，因为其生产能力高（4.03 mU mL(-1) h(-1)）。
