[免疫印迹法检测人血清中抗弓形虫抗体]
[Detection of antibodies against Toxoplasma gondii in human sera in immunoblot procedures].
作者信息
Koch G, Wohlfahrth A, Müller W A
机构信息
Institut für Medizinische Mikrobiologie, Medizinischen Akademie Magdeburg.
出版信息
Z Gesamte Hyg. 1990 Oct;36(10):555-8.
PMID:2284822
Abstract
Proteins of Toxoplasma lysate were separated by SDS-polyacrylamide gel electrophoresis with subsequent transfer to a nitrocellulose sheet by electrophoretic blotting. Patient human sera reacted predominant with protein fractions of Toxoplasma antigen of 94 kD and 35 kD. Human sera were tested by Toxoplasma-IgG ELISA and Immunofluorescence assay (IgG-IFAT, IgM-IFAT).
摘要
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离弓形虫裂解物的蛋白质，随后通过电泳印迹将其转移到硝酸纤维素膜上。患者血清主要与94kD和35kD的弓形虫抗原蛋白组分发生反应。通过弓形虫IgG酶联免疫吸附测定法和免疫荧光试验（IgG免疫荧光抗体试验、IgM免疫荧光抗体试验）检测人血清。
Zotero 插件