[抗弓形虫单克隆抗体的特性研究]
[Characterization of monoclonal antibodies to Toxoplasma gondii].
作者信息
Koch G, Wohlfarth A, Müller W A
出版信息
Z Gesamte Hyg. 1989 Oct;35(10):615-7.
PMID:2694652
Abstract
Proteins of Toxoplasma lysate were separated by SDS-polyacrylamide gel electrophoresis with subsequent transfer to a nitrocellulose sheet by electrophoretic blotting. The range of protein bands was 6-100 kD. Monoclonal antibodies 1B2, 5B10, 4F6 reacted with antigens of 21 kD, 35 kD and 66 kD. Monoclonal antibodies reacted with major antigens of the RH-tachyzoiten surface and HanR-cysts.
摘要
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离弓形虫裂解物的蛋白质，随后通过电泳印迹法将其转移到硝酸纤维素膜上。蛋白质条带范围为6 - 100 kD。单克隆抗体1B2、5B10、4F6与21 kD、35 kD和66 kD的抗原发生反应。单克隆抗体与速殖子RH株表面及缓殖子HanR株包囊的主要抗原发生反应。
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