Mohammed N, Rodriguez M, Garcia V, Garcia J M, Dominguez G, Peña C, Herrera M, Gomez I, Diaz R, Soldevilla B, Herrera A, Silva J, Bonilla F
Department of Medical Oncology, University Hospital Puerta de Hierro Majadahonda, E-28222 Madrid, Spain.
Oncol Lett. 2011 Jul;2(4):719-724. doi: 10.3892/ol.2011.294. Epub 2011 Apr 28.
The presence of free nucleic acids in plasma has been detected in cancer patients and is associated with poor prognosis. In the present study, the mRNA levels of three genes (EPAS1, KIAA0101 and UBE2D3) in plasma from colorectal cancer patients were analyzed. These genes were selected from a previous study of genomic profiles, discriminating between healthy controls and colorectal cancer patients. mRNA levels were analyzed by real-time PCR in the plasma of 154 patients with colorectal cancer. The association of plasma mRNA levels with clinicopathological parameters and patient survival were analyzed. High levels of EPAS1 in the plasma were associated with patients aged over 50 years, relapse of disease and patient mortality. When patients were divided into two groups, early (I and II) and advanced (III and IV) stages, an association was observed between high levels of EPAS1 mRNA and worse disease-free and overall survival in advanced stages. The expression of KIAA0101 and UBE2D3 was not associated with poor prognosis. Thus, our results suggest that EPAS1 mRNA levels may be an indicator of poor prognosis in colorectal cancer patients at advanced stages, obtained by a non-invasive method.
在癌症患者中已检测到血浆中存在游离核酸，且其与预后不良相关。在本研究中，分析了来自结直肠癌患者血浆中三个基因（EPAS1、KIAA0101和UBE2D3）的mRNA水平。这些基因是从先前一项关于基因组图谱的研究中挑选出来的，用于区分健康对照者和结直肠癌患者。通过实时PCR分析了154例结直肠癌患者血浆中的mRNA水平。分析了血浆mRNA水平与临床病理参数及患者生存情况的相关性。血浆中EPAS1水平高与年龄超过50岁的患者、疾病复发及患者死亡相关。当将患者分为早期（I期和II期）和晚期（III期和IV期）两组时，观察到EPAS1 mRNA水平高与晚期患者较差的无病生存期和总生存期之间存在关联。KIAA0101和UBE2D3的表达与预后不良无关。因此，我们的结果表明，EPAS1 mRNA水平可能是晚期结直肠癌患者预后不良的一个指标，可通过非侵入性方法获得。
