Iida Hiroya, Hata Masaki, Kakuno Ayako, Hirano Hiroshi, Yamanegi Koji, Yamada Naoko, Ohyama Hideki, Terada Nobuyuki, Yasui Chiaki, Yamanaka Naoki, Nakasho Keiji
Department of Pathology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501.
Oncol Lett. 2011 Nov;2(6):1041-1046. doi: 10.3892/ol.2011.405. Epub 2011 Sep 2.
In this study, the expression of hepatocyte markers, including α-fetoprotein (AFP), HepPar-1 antigen and arginase-1, was examined immunohistochemically in 14 mass-forming peripheral intrahepatic cholangiocarcinomas (ICCs) that arose from the peripheral portion of the biliary tree, and in 14 periductal-infiltrating hilar ICCs that arose from intrahepatic large bile ducts. Only 2 (14.3%) of the 14 hilar ICCs and 2 (14.3%) of the 14 peripheral ICCs expressed AFP or HepPar-1 antigen. Conversely, arginase-1 was expressed in 8 (57.1%) and 11 (78.6%) of the hilar and peripheral ICCs, respectively, and 4 (28.6%) hilar ICCs and 7 (50%) peripheral ICCs expressed arginase-1 in more than 10% of the cancer cells. The expression of arginase-1 did not differ between peripheral ICCs showing major histology of poorly differentiated adenocarcinoma and those showing other major histologies, including well-or moderately differentiated tubular adenocarcinoma or papillary adenocarcinoma. Results of the present study showed that common hepatocyte markers, including AFP and HepPar-1 antigen, are rarely but definitely expressed in hilar and peripheral ICCs, and that a third hepatocyte marker, arginase-1, is expressed at a high rate in both hilar and peripheral ICCs, irrespective of their histology. These results indicate that care should be taken when using arginase-1 as a hepatocyte marker for distinguishing between a poorly differentiated hepatocellular carcinoma and a mass-forming peripheral ICC showing the histology of poorly differentiated adenocarcinoma.
在本研究中，采用免疫组织化学方法检测了14例起源于肝外胆管周围部分的肿块型肝内胆管癌（ICC）以及14例起源于肝内大胆管的肝门部ICC中肝细胞标志物的表达情况，这些标志物包括甲胎蛋白（AFP）、HepPar-1抗原和精氨酸酶-1。14例肝门部ICC中仅有2例（14.3%）、14例周围型ICC中仅有2例（14.3%）表达AFP或HepPar-1抗原。相反，精氨酸酶-1分别在8例（57.1%）肝门部ICC和11例（78.6%）周围型ICC中表达，4例（28.6%）肝门部ICC和7例（50%）周围型ICC中超过10%的癌细胞表达精氨酸酶-1。在主要组织学类型为低分化腺癌的周围型ICC与主要组织学类型为其他类型（包括高分化或中分化管状腺癌或乳头状腺癌）的周围型ICC之间，精氨酸酶-1的表达无差异。本研究结果表明，包括AFP和HepPar-1抗原在内的常见肝细胞标志物在肝门部和周围型ICC中很少表达，但确实有表达，并且第三种肝细胞标志物精氨酸酶-1在肝门部和周围型ICC中均有较高表达，与它们的组织学类型无关。这些结果表明，在使用精氨酸酶-1作为肝细胞标志物来区分低分化肝细胞癌和表现为低分化腺癌组织学类型的肿块型周围型ICC时应谨慎。
