冬凌草甲素诱导SW620人结肠腺癌细胞凋亡
Oridonin-induced apoptosis in SW620 human colorectal adenocarcinoma cells.
作者信息
Ji Zhe, Tang Qingjiu, Zhang Jinsong, Yang Yan, Liu Yanfang, Pan Yingjie
机构信息
National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201106.
出版信息
Oncol Lett. 2011 Nov;2(6):1303-1307. doi: 10.3892/ol.2011.408. Epub 2011 Sep 2.
Abstract
Oridonin, a diterpenoid isolated from Rabdosia rubescens (Hemsl.) Hara, inhibited the growth of human tumor cell lines SW620 (colon), MCF-7 (breast) and K562 (bone marrow), and induced significant levels of apoptosis in SW620. Morphological changes indicative of cell apoptosis were observed after the cells were exposed to oridonin for 24 h. Growth inhibition was associated with G1 phase arrest, and with time- and dose-dependent increases in caspase-3 activity. We therefore conclude that oridonin inhibits the proliferation of SW620 cells by induction of apoptosis via the activation of caspase-3. Our data suggest that oridonin may have significant potential as an anti-colorectal adenocarcinoma agent.
摘要
冬凌草甲素是从冬凌草中分离得到的一种二萜类化合物，它能够抑制人肿瘤细胞系SW620（结肠）、MCF-7（乳腺）和K562（骨髓）的生长，并在SW620细胞中诱导显著水平的细胞凋亡。细胞暴露于冬凌草甲素24小时后，观察到了指示细胞凋亡的形态学变化。生长抑制与G1期阻滞相关，并伴随caspase-3活性的时间和剂量依赖性增加。因此，我们得出结论，冬凌草甲素通过激活caspase-3诱导细胞凋亡来抑制SW620细胞的增殖。我们的数据表明，冬凌草甲素作为一种抗结肠腺癌药物可能具有巨大潜力。
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