Department of Histology, Universitas Indonesia, Jakarta, Indonesia.
Curr Stem Cell Res Ther. 2012 Sep;7(5):329-35. doi: 10.2174/157488812802481508.
Stem cell culture for regenerative medicine needs platelet rich plasma (PRP) as fetal bovine/calf serum (FBS/FCS) substitute. However, the various studies used various protocols in preparing and processing the PRP. This study aimed to compare and conclude the most effective and efficient protocol.
we searched in vitro studies that used human PRP as FBS/FCS substitute to culture human cells, and compared the various available protocols to identify the easiest and effective protocols for the preparation of PRP and the release of the growth factors (GFs) to support the highest cell growth in stem cell culture.
ten studies fulfilled the selection criteria and were included in the analysis.
Almost all studies on bone marrow mesenchymal stem cell (BM-MSC) and adipose stem cell (AT-SC) showed that platelet lysate and/or activated platelet releasate were superior or at least the same as either FBS or FCS, except for one study that got different results on human AT-SC. Several studies showed that either 5% activated PRP (aPRP) or platelet lysate (PL) was sufficient to support cell growth, or even better when they were compared to 10% FBS, while higher concentrations were counterproductive. However, some studies showed that 10% aPRP or PL was needed. The difference between studies was due to the difference in either the PRP preparation from blood and in the PRP processing to release the GFs, which yield various GF concentrations.
In conclusion, studies are needed to reveal the optimal final platelet counts for the various PRP processing methods for various kinds of cells. The easiest PRP processing is freezing to -20°C followed by thawing, or thrombin activation using a final concentration of 100U/mL.
干细胞培养再生医学需要富血小板血浆（PRP）作为胎牛/小牛血清（FBS/FCS）的替代品。然而，各种研究在制备和处理 PRP 时使用了不同的方案。本研究旨在比较和总结最有效和最有效的方案。
我们搜索了使用人 PRP 作为 FBS/FCS 替代品培养人细胞的体外研究，并比较了各种可用方案，以确定制备 PRP 和释放生长因子（GFs）的最简单和最有效的方案，以支持干细胞培养中细胞的最高生长。
符合选择标准的 10 项研究被纳入分析。
几乎所有关于骨髓间充质干细胞（BM-MSC）和脂肪干细胞（AT-SC）的研究都表明，血小板裂解物和/或激活的血小板释放物优于或至少与 FBS 或 FCS 相同，除了一项研究在人类 AT-SC 上得到了不同的结果。几项研究表明，5%激活的 PRP（aPRP）或血小板裂解物（PL）足以支持细胞生长，甚至比 10% FBS 更好，而更高的浓度则适得其反。然而，一些研究表明需要 10% aPRP 或 PL。研究之间的差异是由于血液中 PRP 的制备以及释放 GFs 的 PRP 处理方式的差异，这导致了各种 GF 浓度的差异。
总之，需要进行研究以揭示各种 PRP 处理方法对各种细胞的最佳最终血小板计数。最简单的 PRP 处理是冷冻至-20°C，然后解冻，或使用最终浓度为 100U/mL 的凝血酶激活。
