REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal.
J Agric Food Chem. 2012 Aug 22;60(33):8103-10. doi: 10.1021/jf302898z. Epub 2012 Aug 13.
Hazelnut is one of the most commonly consumed tree nuts, being largely used by the food industry in a wide variety of processed foods. However, it is a source of allergens capable of inducing mild to severe allergic reactions in sensitized individuals. Hence, the development of highly sensitive methodologies for hazelnut traceability is essential. In this work, we developed a novel technique for hazelnut detection based on a single-tube nested real-time PCR system. The system presents high specificity and sensitivity, enabling a relative limit of detection of 50 mg/kg of hazelnut in wheat material and an absolute limit of detection of 0.5 pg of hazelnut DNA (1 DNA copy). Its application to processed food samples was successfully achieved, detecting trace amounts of hazelnut in chocolate down to 60 mg/kg. These results highlight the adequacy of the technique for the specific detection and semiquantitation of hazelnut as potential hidden allergens in foods.
榛子是最常食用的坚果之一，在食品工业中广泛用于各种加工食品。然而，它也是过敏原的来源，能够在致敏个体中引起轻度至重度过敏反应。因此，开发高度敏感的榛子溯源方法至关重要。在这项工作中，我们开发了一种基于单管嵌套实时 PCR 系统的榛子检测新技术。该系统具有高特异性和灵敏度，能够在小麦材料中检测到榛子的相对检出限为 50mg/kg，榛子 DNA（1 个 DNA 拷贝）的绝对检出限为 0.5pg。它成功应用于加工食品样品的检测，可检测到巧克力中低至 60mg/kg 的榛子痕量。这些结果表明，该技术适用于特定检测和半定量分析食品中的潜在隐藏过敏原榛子。
