Lima Mônica Cruvinel de, Marks Guido, Silva Iandara Schettert, Silva Baldomero Antonio Kato da, Cônsolo Lourdes Zélia Zanoni, Nogueira Gabriel Bogalho
UFMS, Campo Grande, MS, Brazil.
Acta Cir Bras. 2012 Aug;27(8):544-51. doi: 10.1590/s0102-86502012000800005.
To evaluate the influence of aerobic exercise on oxidative stress in mice.
The study included twenty female mice Mus musculus-Swiss divided into two groups: sedentary control (GA) and exercise (GB), each containing ten animals. All animals underwent an adaptation period of seven days isolated in individual boxes. After this period, the animals in the exercise group (GB) were trained in angled running wheel with circumference of 25 cm assembled on an articulated axle during five minutes for three consecutive days. On the fourth day, they underwent an exercise program of one session lasting 45 minutes. The evaluation of oxidative stress was performed by determining the levels of malondialhyde derived of lipid peroxidation by the TBA method. The samples were read in a spectrophotometer at 535 nm.
No significant difference was observed in the intergroup comparison of MDA levels in the tissues evaluated. A significant difference was observed in the intragroup comparison of MDA levels in the control group (p = 0.0201).The Tukeys' post hoc test indicated significantly lower values of MDA in the smooth muscle in relation to plasma. In the analysis of variance in the exercise group, a significant difference between tissues (p = 0.0009), with significantly lower values in the smooth muscle in relation to plasma (p<0.001) and higher in striated muscle in relation to smooth muscle (p<0.05) was observed.
There was no change in the analysis of oxidative stress in mice which were undergone a single session of aerobic exercise.
评估有氧运动对小鼠氧化应激的影响。
该研究纳入了20只雌性瑞士小鼠，分为两组：久坐对照组（GA）和运动组（GB），每组10只动物。所有动物在单独的笼子里进行了7天的适应期。在此期间后，运动组（GB）的动物在一个周长为25厘米、安装在铰接轴上的倾斜跑步轮上连续三天每天训练5分钟。第四天，它们进行了一次持续45分钟的运动程序。通过TBA法测定脂质过氧化衍生的丙二醛水平来评估氧化应激。样品在535nm处用分光光度计读取。
在所评估的组织中，组间丙二醛水平比较未观察到显著差异。对照组丙二醛水平的组内比较观察到显著差异（p = 0.0201）。Tukeys事后检验表明，平滑肌中的丙二醛值相对于血浆显著较低。在运动组的方差分析中，观察到组织间存在显著差异（p = 0.0009），平滑肌中的值相对于血浆显著较低（p<0.001），而横纹肌相对于平滑肌较高（p<0.05）。
进行单次有氧运动的小鼠氧化应激分析无变化。
