Department of Pharmacology, Clinical Pharmacology and Algesiology, Faculty of Medicine, 'Grigore T. Popa' University of Medicine and Pharmacy, Universitatii St. 16, 700115 Iasi, Romania.
Department of Pharmaceutical Technology, Faculty of Pharmacy, 'Grigore T. Popa' University of Medicine and Pharmacy, Universitatii St. 16, 700115 Iasi, Romania.
Medicina (Kaunas). 2022 Nov 10;58(11):1620. doi: 10.3390/medicina58111620.
: Diabetes mellitus (DM) is a complex disease affecting the whole metabolic balance of the body and resulting in multiple organ complications: cardiovascular, neuronal, renal, etc. Our study focuses on investigating the effect of zinc chloride (Zn) on certain blood parameters suggestive for assessing the metabolic disturbances, the liver and kidney function, the oxidative stress and the immune defense capacity in experimental-induced DM with streptozotocin (STZ) and cholesterol in rats. : The animals were assigned to three groups, as follows: Group 1 (Control): buffer citrate solution 0.1 mL/100 g body; Group 2 (STZ): 20 mg/kg body STZ and fat diet (10 g cholesterol/100 g diet); Group 3 (STZ+Zn): 20 mg/kg body STZ + 5 mg/kg body Zn chloride and the same fat diet. DM was induced by administering STZ in a single take daily, for three consecutive days, Zn and citrate buffer were administered orally for a month. The protocol was approved by the Ethics Committee of the University 'Grigore T Popa' Iasi, in agreement with the International Regulations about the handling of laboratory animals. The use of STZ in rats fed with cholesterol was correlated with important weight gain, hyperglycemia, the intensification of the transaminases activity and the increase in serum alkaline phosphatase, cholesterol, triglyceride, urea, creatinine and in malondialdehyde. : The treatment with Zn resulted in weight loss and a decrease in blood sugar in diabetic rats. Supplementation with Zn notably reduced oxidative stress, preserved the pancreatic architecture and restored the liver and kidney function and structure in STZ-induced DM in rats.
糖尿病（DM）是一种影响全身代谢平衡的复杂疾病，会导致多种器官并发症：心血管、神经元、肾脏等。我们的研究重点是研究氯化锌（Zn）对某些血液参数的影响，这些参数提示评估代谢紊乱、肝功能、肾功能、氧化应激和免疫防御能力在链脲佐菌素（STZ）和胆固醇诱导的实验性糖尿病大鼠。
动物分为三组，如下：
组 1（对照）：柠檬酸缓冲液 0.1 mL/100 g 体重；
组 2（STZ）：20 mg/kg 体重 STZ 和高脂肪饮食（10 g 胆固醇/100 g 饮食）；
组 3（STZ+Zn）：20 mg/kg 体重 STZ + 5 mg/kg 体重 Zn 氯化物和相同的高脂肪饮食。DM 通过连续三天每天给予一次 STZ 诱导，Zn 和柠檬酸缓冲液通过口服给予一个月。该方案得到了雅西“Grigore T Popa”大学伦理委员会的批准，符合实验室动物处理的国际法规。
在喂食胆固醇的大鼠中使用 STZ 与体重显著增加、高血糖、转氨酶活性增强以及血清碱性磷酸酶、胆固醇、甘油三酯、尿素、肌酐和丙二醛增加有关。
用 Zn 治疗可导致糖尿病大鼠体重减轻和血糖降低。Zn 补充剂显著减轻氧化应激，保护胰腺结构，并恢复 STZ 诱导的 DM 大鼠的肝功能和肾功能及结构。
