Gaspareto Karine Vieira, Mello Flávia Myrian Martins de Almeida, Dias José Ricardo Colleti, Meneguetti Vera Alice Fernandes, Storti Marta Evelyn Giansante, Ferreira João Leandro de Paula, Lança André Minhoto, Rodrigues Rosângela, Brígido Luis Fernando de Macedo, Teixeira Jorge Juarez Vieira, Bertolini Dennis Armando
Laboratório de Retrovírus, Centro de Virologia, Instituto Adolfo Lutz, São Paulo, SP, Brazil.
Rev Inst Med Trop Sao Paulo. 2012 Aug;54(4):207-13. doi: 10.1590/s0036-46652012000400005.
The objective of this study is to identify subtypes of Human Immunodeficiency Virus type 1 (HIV-1) and to analyze the presence of mutations associated to antiretroviral resistance in the protease (PR) and reverse transcriptase (RT) regions from 48 HIV-1 positive treatment naïve patients from an outpatient clinic in Maringá, Paraná, Brazil. Sequencing was conducted using PR, partial RT and group-specific antigen gene (gag) nested PCR products from retrotranscribed RNA. Transmitted resistance was determined according to the Surveillance Drug Resistance Mutation List (SDRM) algorithm. Phylogenetic and SimPlot analysis of concatenated genetic segments classified sequences as subtype B 19/48 (39.6%), subtype C 12/48 (25%), subtype F 4/48 (8.3%), with 13/48 (27.1%) recombinant forms. Most recombinant forms were B mosaics (B/F 12.5%, B/C 10.4%), with one C/F (2.1%) and one complex B/C/F mosaic (2.1%). Low levels of transmitted resistance were found in this study, 2/48 (2.1% to NRTIs and 2.1% for PI). This preliminary data may subsidize the monitoring of the HIV evolution in the region.
本研究的目的是鉴定1型人类免疫缺陷病毒（HIV-1）的亚型，并分析来自巴西巴拉那州马林加市一家门诊诊所的48名初治HIV-1阳性患者蛋白酶（PR）和逆转录酶（RT）区域中与抗逆转录病毒耐药性相关的突变情况。使用逆转录RNA的PR、部分RT和组特异性抗原基因（gag）巢式PCR产物进行测序。根据监测耐药突变列表（SDRM）算法确定传播耐药性。对串联基因片段进行系统发育和SimPlot分析，将序列分类为B亚型19/48（39.6%）、C亚型12/48（25%）、F亚型4/48（8.3%），有13/48（27.1%）为重组形式。大多数重组形式为B型镶嵌体（B/F 12.5%，B/C 10.4%），有一个C/F（2.1%）和一个复杂的B/C/F镶嵌体（2.1%）。本研究发现传播耐药性水平较低，2/48（对核苷类逆转录酶抑制剂为2.1%，对蛋白酶抑制剂为2.1%）。这些初步数据可能有助于该地区HIV演变的监测。
