Dell M P, Haswell S J, Roch O G, Coker R D, Medlock V F, Tomlins K
School of Chemistry, Thames Polytechnic, London, UK.
Analyst. 1990 Nov;115(11):1435-9. doi: 10.1039/an9901501435.
High-performance thin-layer chromatography (HPTLC) was applied to the separation and quantification of aflatoxin in 300 jars of "crunchy" peanut butter. A critical evaluation of the proposed HPTLC method has been carried out by statistical comparisons with a commercially available enzyme-linked immunosorbent assay (ELISA) kit and a high-performance liquid chromatographic (HPLC) method. The statistical tests indicated that whilst the distributions of the data sets obtained with each method were similar, the HPLC method was found to be biased. Over-all results indicated that the HPTLC method gave more consistent data, relatively lower standard deviations and lower coefficients of variation. The ELISA kit was found to be less precise than the HPTLC and HPLC methods and prone to some loss of sensitivity caused by matrix interference.
采用高效薄层色谱法（HPTLC）对300罐“脆”花生酱中的黄曲霉毒素进行分离和定量。通过与市售酶联免疫吸附测定（ELISA）试剂盒和高效液相色谱（HPLC）方法进行统计比较，对所提出的HPTLC方法进行了严格评估。统计测试表明，虽然每种方法获得的数据集分布相似，但发现HPLC方法存在偏差。总体结果表明，HPTLC方法给出的数据更一致，相对标准偏差较低，变异系数也较低。发现ELISA试剂盒不如HPTLC和HPLC方法精确，并且容易因基质干扰而导致一些灵敏度损失。
