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肉瘤与间充质干细胞中基因表达谱的比较确定了化学诱导大鼠肉瘤模型中的致瘤途径。

Comparison of gene expression profiling in sarcomas and mesenchymal stem cells identifies tumorigenic pathways in chemically induced rat sarcoma model.

作者信息

Honoki Kanya, Fujii Hiromasa, Tohma Yasuaki, Tsujiuchi Toshifumi, Kido Akira, Tsukamoto Shinji, Mori Toshio, Tanaka Yasuhito

机构信息

Department of Orthopedic Surgery, Nara Medical University, 840 Shijo-cho, Kashihara 634-8521, Japan.

出版信息

ISRN Oncol. 2012;2012:909453. doi: 10.5402/2012/909453. Epub 2012 Jul 17.

DOI:10.5402/2012/909453
PMID:22852096
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3407640/
Abstract

Mesenchymal stem cells (MSCs) are believed to be the cell of origin for most sarcomas including osteosarcoma and malignant fibrous histiocytoma (MFH/UPS). To identify the signaling pathways involved in sarcoma pathogenesis, we compared gene expression profiles in rat osteosarcoma and MFH cells with those in syngeneic rat MSCs. Analysis of genes that characterize MSCs such as CD44, CD105, CD73, and CD90 showed higher expression in MSCs compared to sarcomas. Pathways involved in focal and cell adhesion, cytokine-cytokine receptors, extracellular matrix receptors, chemokines, and Wnt signaling were down-regulated in both sarcomas. Meanwhile, DNA replication, cell cycle, mismatch repair, Hedgehog signaling, and metabolic pathways were upregulated in both sarcomas. Downregulation of p21(Cip1) and higher expression of CDK4-cyclinD1 and CDK2-cyclinE could accelerate cell cycle in sarcomas. The current study indicated that these rat sarcomas could be a good model for their human counterparts and will provide the further insights into the molecular pathways and mechanisms involved in sarcoma pathogenesis.

摘要

间充质干细胞（MSCs）被认为是包括骨肉瘤和恶性纤维组织细胞瘤（MFH/UPS）在内的大多数肉瘤的起源细胞。为了确定参与肉瘤发病机制的信号通路，我们比较了大鼠骨肉瘤和MFH细胞与同基因大鼠MSCs的基因表达谱。对表征MSCs的基因（如CD44、CD105、CD73和CD90）的分析表明，与肉瘤相比，这些基因在MSCs中的表达更高。在两种肉瘤中，参与粘着斑和细胞粘附、细胞因子-细胞因子受体、细胞外基质受体、趋化因子和Wnt信号传导的通路均下调。同时，两种肉瘤中DNA复制、细胞周期、错配修复、Hedgehog信号传导和代谢通路均上调。p21（Cip1）的下调以及CDK4-细胞周期蛋白D1和CDK2-细胞周期蛋白E的高表达可加速肉瘤中的细胞周期。目前的研究表明，这些大鼠肉瘤可能是其人类对应物的良好模型，并将为深入了解肉瘤发病机制中涉及的分子途径和机制提供进一步的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/b2fc4bf8f0a7/ISRN.ONCOLOGY2012-909453.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/02a90788c052/ISRN.ONCOLOGY2012-909453.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/5bc931a31b52/ISRN.ONCOLOGY2012-909453.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/d7fbb00e23de/ISRN.ONCOLOGY2012-909453.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/c58d93b92c61/ISRN.ONCOLOGY2012-909453.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/497062d81dd5/ISRN.ONCOLOGY2012-909453.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/09708ec67b57/ISRN.ONCOLOGY2012-909453.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/074dfa40a36d/ISRN.ONCOLOGY2012-909453.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/b2fc4bf8f0a7/ISRN.ONCOLOGY2012-909453.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/02a90788c052/ISRN.ONCOLOGY2012-909453.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/5bc931a31b52/ISRN.ONCOLOGY2012-909453.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/d7fbb00e23de/ISRN.ONCOLOGY2012-909453.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/c58d93b92c61/ISRN.ONCOLOGY2012-909453.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/497062d81dd5/ISRN.ONCOLOGY2012-909453.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/09708ec67b57/ISRN.ONCOLOGY2012-909453.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/074dfa40a36d/ISRN.ONCOLOGY2012-909453.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/201e/3407640/b2fc4bf8f0a7/ISRN.ONCOLOGY2012-909453.008.jpg

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2
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Cancer Cell. 2011 May 17;19(5):601-12. doi: 10.1016/j.ccr.2011.03.010.
3
Role of GLI2 in the growth of human osteosarcoma.GLI2 在人骨肉瘤生长中的作用。
β-连环蛋白的转录活性在犬骨肉瘤中极低，对其进行靶向抑制后，细胞系行为的变化也极小。
Vet Comp Oncol. 2016 Jun;14(2):e4-e16. doi: 10.1111/vco.12077. Epub 2013 Nov 21.
4
Histologic and genetic advances in refining the diagnosis of "undifferentiated pleomorphic sarcoma".在“未分化多形性肉瘤”的诊断中，组织学和遗传学的进步。
Cancers (Basel). 2013 Feb 22;5(1):218-33. doi: 10.3390/cancers5010218.
5
Lost miRNA surveillance of Notch, IGFR pathway--road to sarcomagenesis.Notch、IGFR 通路的微小 RNA 监测缺失——通往肉瘤发生之路
Tumour Biol. 2014 Jan;35(1):483-92. doi: 10.1007/s13277-013-1068-5.
J Pathol. 2011 Jun;224(2):169-79. doi: 10.1002/path.2880. Epub 2011 Apr 19.
4
Rb regulates fate choice and lineage commitment in vivo.Rb 调节体内命运选择和谱系承诺。
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