Floridi A, Gentile F P, Bruno T, Castiglione S, Zeuli M, Benassi M
Laboratory for Cell Metabolism and Pharmacokinetics, Regina Elena Institute for Cancer Research, Rome, Italy.
Anticancer Res. 1990 Nov-Dec;10(6):1633-6.
The effect of Rhein (RH) and Lonidamine (LND) on the clonogenic activity of cultured human glioma cells has been evaluated. Both these drugs decrease the survival fraction, but their effect is strictly related to the duration of exposure. A brief exposure, i.e. 4 hours, even at the highest drug concentrations does not induce any significant decrease in the survival which, on the contrary, is strongly affected by 24 and 48 hours of exposure. The reason for this behaviour lies in the mechanism of action of these drugs which do not interfere with replicative processes, but selectively affect the energy metabolism of the neoplastic cell. The validity of currently employed screening tests to evaluate the antitumoral activity of non anti-mitotic drugs is also discussed.
已评估了大黄酸（RH）和氯尼达明（LND）对培养的人胶质瘤细胞克隆形成活性的影响。这两种药物均降低了存活分数，但其效果与暴露持续时间密切相关。短暂暴露，即4小时，即使在最高药物浓度下也不会导致存活率有任何显著下降，相反，24小时和48小时的暴露会强烈影响存活率。这种现象的原因在于这些药物的作用机制，它们不干扰复制过程，而是选择性地影响肿瘤细胞的能量代谢。还讨论了当前用于评估非抗有丝分裂药物抗肿瘤活性的筛选试验的有效性。
