An Jeung Hee, El-Said Waleed Ahmed, Choi Jeong-Woo
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, 121-742, Korea.
J Nanosci Nanotechnol. 2012 May;12(5):4115-8. doi: 10.1166/jnn.2012.5903.
Oxidative stress has been implicated in pesticide-induced neurotoxicity, base on its role in cascade of biochemical changes that lead to dopaminergic neuronal cell death. The present study examined the role of oxidative stress and the electrochemical detection by polychlorinated biphenyls (PCBs)-induced toxicant in SH-SY5Y cell. The cells were seed in the RED (Arg-Gly-Asp) nanopatterned coating gold substrate and treated with different concentration of PCBs for 24 h in culture, which induced the change of the cyclic voltammetry (CV) current peak. The CV results showed that PCB significantly decreased the current peaks in dose and time-dependent manner. After antioxidant treatment, the CV of the PCB-treated cell chip increased the current peak. Especially, gluthaione and catalase prevent PCB-induced decrease of CV current peak in the cell. The results demonstrated that the current peak decreased by the PCB and recovered by the antioxidant enzyme. In conclusion, results suggest that the electrochemical-based chip provide crucial information to improvement toward a cell chip system for drug screening application.
氧化应激因其在导致多巴胺能神经元细胞死亡的一系列生化变化中的作用，而与农药诱导的神经毒性有关。本研究考察了氧化应激的作用以及多氯联苯（PCBs）诱导的毒物在SH-SY5Y细胞中的电化学检测。将细胞接种于RED（精氨酸-甘氨酸-天冬氨酸）纳米图案化涂层金基底上，并在培养中用不同浓度的PCBs处理24小时，这导致了循环伏安法（CV）电流峰的变化。CV结果表明，PCBs以剂量和时间依赖性方式显著降低电流峰。抗氧化剂处理后，经PCBs处理的细胞芯片的CV增加了电流峰。特别是，谷胱甘肽和过氧化氢酶可防止PCBs诱导的细胞中CV电流峰降低。结果表明，PCBs使电流峰降低，而抗氧化酶使其恢复。总之，结果表明基于电化学的芯片为改进用于药物筛选应用的细胞芯片系统提供了关键信息。
