Department of Chemical Engineering, Research Center of Chemical Technology, Hankyong National University, Anseong, Gyeonggi-Do 456-749, Republic of Korea.
J Biomed Nanotechnol. 2013 May;9(5):880-5. doi: 10.1166/jbn.2013.1497.
The aim of this study was to develop a microfluidic neuronal cell chip device to monitor the toxic effects of polychlorinated biphenyls (PCBs) on PC-12 neuronal cells. PCBs adversely influence the activities of neuronal cells in the nervous system. In PC-12 cells, the production and secretion of dopamine decreases in response to PCB exposure. The microfluidic device that we developed to measure the amount of dopamine by cyclic voltammetry is composed of a control layer, a fluidic layer, and a gold electrode-patterned glass wafer. The control channel in the control layer functions as a microvalve to control the flow of the fluidic channel in the fluidic layer. The fluidic layer consists of 3 reaction chambers as well as fluidic channels. Three electrodes, including the working electrode, counter electrodes, and a reference electrode, are placed in a fluidic chamber. The electrochemical signals of dopamine, either from a standard dopamine solution or from the culture supernatant from cultured PC-12 cells, were obtained using a fabricated microfluidic neuronal cell chip by cyclic voltammetry. When PCBs were added to cultures of PC-12 cells, the amount of dopamine secreted from the PC-12 cells decreased due to the reduced activity of PC-12 cells. The fabricated neuronal cell chip was capable of detecting the toxic effect of dopamine on neuronal cells at concentrations of 10 microg/L and over. The practicality of the developed microfluidic neuronal cell chip was validated using river water spiked with PCBs.
本研究旨在开发一种微流控神经元细胞芯片装置，以监测多氯联苯 (PCBs) 对 PC-12 神经元细胞的毒性作用。PCBs 会对神经系统中神经元细胞的活动产生不利影响。在 PC-12 细胞中，PCB 暴露会导致多巴胺的产生和分泌减少。我们开发的用于通过循环伏安法测量多巴胺量的微流控装置由控制层、流道层和金电极图案化玻璃晶片组成。控制层中的控制通道作为微阀，用于控制流道层中的流体通道的流动。流道层由 3 个反应室和流道组成。三个电极，包括工作电极、对电极和参比电极，放置在一个流道室中。通过循环伏安法，使用制造的微流控神经元细胞芯片获得来自标准多巴胺溶液或培养的 PC-12 细胞培养上清液的多巴胺的电化学信号。当将 PCBs 添加到 PC-12 细胞的培养物中时，由于 PC-12 细胞的活性降低，从 PC-12 细胞分泌的多巴胺量减少。制造的神经元细胞芯片能够检测出多巴胺对神经元细胞的毒性作用，其浓度为 10μg/L 及以上。使用含 PCB 的河水验证了所开发的微流控神经元细胞芯片的实用性。
