Chen L H
Department of Nutrition and Food Science, Lexington, Kentucky 40506-0054.
Anticancer Res. 1990 Nov-Dec;10(6):1707-10.
The effect of butylated hydroxyanisole (BHA) on the activity of glutathione-S-transferase (GSH-S-T) in hepatocyte culture was determined. Hepatocytes from control rats and rats injected with 3-methylcholanthrene (3-MC) were isolated and cultured. The cultures were treated with various concentrations of BHA. Treatment of hepatocytes from both control and 3-MC-injected rats with 25 microM BHA for 24 or 48 hours increased GSH-S-T activity significantly. The results suggest that BHA may have a detoxifying effect against 3-MC-induced carcinogenesis, and that hepatocyte culture may be a fast and simple system to identify compounds which may potentially protect against development of cancer through induction of GSH-S-T.
测定了丁基羟基茴香醚（BHA）对肝细胞培养物中谷胱甘肽-S-转移酶（GSH-S-T）活性的影响。分离并培养了来自对照大鼠和注射了3-甲基胆蒽（3-MC）的大鼠的肝细胞。用不同浓度的BHA处理培养物。用25 microM BHA处理对照大鼠和注射3-MC大鼠的肝细胞24或48小时后，GSH-S-T活性显著增加。结果表明，BHA可能对3-MC诱导的致癌作用具有解毒作用，并且肝细胞培养可能是一种快速简便的系统，用于鉴定可能通过诱导GSH-S-T潜在预防癌症发生的化合物。
