Zakhary Nadia Iskander, Boshra Sylvia Azmy, El-Sawalhi Maha Mahmoud, Fahim Atef Tadros, Ebeid Emad Nabil
Cancer Biology Department, National Cancer Institute, Cairo University, Giza, Egypt.
Genet Test Mol Biomarkers. 2012 Sep;16(9):1067-72. doi: 10.1089/gtmb.2012.0039. Epub 2012 Aug 1.
Insulin-like growth factors (IGFs) and IGF-binding proteins (IGFBPs) have been reported to play an important role in tumor proliferation. This study aimed to investigate the validity of measuring IGFs and specific IGFBPs in the serum of Egyptian children with acute lymphoblastic leukemia (ALL) as additional markers in diagnosis and follow-up of the disease. IGF-I, IGF-II, IGFBP-2, and IGFBP-3 were determined in the sera of 33 ALL patients at time of diagnosis and after an intensification phase of chemotherapy (IPC) that lasts about 6 months as well as in 15 healthy children as a control group using enzyme-linked immunosorbent assay (ELISA) technique. At time of diagnosis, serum IGF-I, IGF-II, and IGFBP-3 were significantly lower than those in the control group. After IPC, serum IGF-I and IGF-II returned to their normal levels, while serum IGFBP-3 was still decreased. On the other hand, serum IGFBP-2 was significantly higher than those in the control group at diagnosis, but returned to normal value after IPC. In conclusion, the changes in IGF system could be useful to support diagnosis and follow-up of children with ALL.
胰岛素样生长因子（IGFs）和胰岛素样生长因子结合蛋白（IGFBPs）据报道在肿瘤增殖中发挥重要作用。本研究旨在调查在埃及急性淋巴细胞白血病（ALL）患儿血清中检测IGFs和特定IGFBPs作为该疾病诊断和随访的额外标志物的有效性。采用酶联免疫吸附测定（ELISA）技术，在33例ALL患者诊断时、持续约6个月的强化化疗阶段（IPC）后以及15名健康儿童作为对照组的血清中测定IGF-I、IGF-II、IGFBP-2和IGFBP-3。诊断时，血清IGF-I、IGF-II和IGFBP-3显著低于对照组。IPC后，血清IGF-I和IGF-II恢复到正常水平，而血清IGFBP-3仍降低。另一方面，血清IGFBP-2在诊断时显著高于对照组，但IPC后恢复到正常数值。总之，IGF系统的变化可能有助于支持ALL患儿的诊断和随访。
