果蝇睾丸的染色质染色。

Chromatin staining of Drosophila testes.

作者信息

Bonaccorsi Silvia, Giansanti Maria G, Cenci Giovanni, Gatti Maurizio

出版信息

Cold Spring Harb Protoc. 2012 Aug 1;2012(8):pdb.prot067363. doi: 10.1101/pdb.prot067363.

Abstract

This protocol describes chromatin staining of Drosophila testes. To visualize DNA, preparations fixed using methanol-acetone, paraformaldehyde, or formaldehyde can be stained with several DNA-binding dyes. If the slides are to be examined with a fluorescence microscope equipped with filters that permit ultraviolet (UV) excitation, suitable dyes for DNA staining are Hoechst 33258 or 4',6-diamidino-2-phenylindole (DAPI). If the slides are to be analyzed with a confocal microscope not equipped with a UV laser, DNA can be stained with either propidium iodide or TOTO-3 iodide.

摘要

本方案描述了果蝇睾丸的染色质染色方法。为了观察DNA，用甲醇-丙酮、多聚甲醛或甲醛固定的样本可以用几种DNA结合染料进行染色。如果要使用配备允许紫外线（UV）激发的滤光片的荧光显微镜检查玻片，适用于DNA染色的染料是Hoechst 33258或4',6-二脒基-2-苯基吲哚（DAPI）。如果要用未配备UV激光的共聚焦显微镜分析玻片，DNA可以用碘化丙啶或碘化TOTO-3进行染色。