Department of Dermatology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan.
Acta Derm Venereol. 2013 Jan;93(1):39-43. doi: 10.2340/00015555-1420.
We investigated the expression of both epidermal fatty acid-binding protein (FABP5), a marker of transit amplifying cells, and nestin, a putative marker of epidermal stem cells, in psoriatic epidermis and in normal human cultured keratinocytes. In lesional psoriatic epidermis, immunostaining showed that the suprabasal layer was positive for nestin, with some cells co-expressing FABP5. Flow cytometric analysis revealed that the expression of both nestin and FABP5 were increased in keratinocytes cultured in a low concentration of calcium relative to those cultured in a high concentration of calcium. These results suggest that nestin and FABP5 are expressed in actively proliferating keratinocytes in vitro and in the suprabasal layer in lesional psoriatic epidermis, and that double-positive cells may identify transit amplifying cells in the epidermis.
我们研究了表皮脂肪酸结合蛋白（FABP5）的表达，它是过渡扩增细胞的标志物，以及巢蛋白，一种表皮干细胞的假定标志物，在银屑病表皮和正常的人培养角质形成细胞中的表达。在病变的银屑病表皮中，免疫染色显示，巢蛋白在上皮细胞层呈阳性，一些细胞同时表达 FABP5。流式细胞术分析显示，与在高浓度钙中培养的角质形成细胞相比，在低浓度钙中培养的角质形成细胞中巢蛋白和 FABP5 的表达均增加。这些结果表明，巢蛋白和 FABP5 在体外活跃增殖的角质形成细胞中和病变银屑病表皮的上皮细胞层中表达，并且双阳性细胞可能鉴定表皮中的过渡扩增细胞。
