Graduate program in Animal Physiology, Faculty of Veterinary Science, Chulalongkorn University, Henri Dunant Road, Patumwan, Bangkok 10330, Thailand.
Vet Res Commun. 2012 Dec;36(4):207-14. doi: 10.1007/s11259-012-9531-0. Epub 2012 Aug 2.
Cardiac autonomic neuropathy in dogs with diabetic mellitus (DM) was evaluated using measurement of heart rate variability (HRV) and plasma norepinephrine (NE) concentration. Dogs were divided into 2 groups; the control non-DM group (n = 13) and the diabetic group (n = 22) which was further divided into the well-controlled DM (n = 11) and the poorly-controlled DM subgroups (n = 11) according to their fasting plasma fructosamine concentrations. The electrocardiogram (ECG) was recorded continuously for at least 30 min to yield HRV. The results showed that in the poorly-controlled DM subgroup, the average of normal R-R interval (mean N-N), SD of the mean of all 5-min segments of normal RR intervals (SDANN) were lower than the control group while heart rate was higher (P < 0.05). The NNA, SDNN, SDNN index and pNN50% were significantly lower when compared with the well-controlled DM subgroup (P < 0.05). The high frequency (HF) and total power were significantly lower while the ratio of low to high frequency (LF/HF) was higher (P < 0.05) when compared with the well-controlled DM subgroup. Moreover, in the poorly-controlled DM subgroup, plasma NE concentration was lower than the control group (210 ± 37 vs. 479 ± 74 pg/ml, P < 0.05). There was a significantly negative correlation between plasma NE and plasma fructosamine concentrations. It is concluded that cardiac autonomic neuropathy occurred in poorly-controlled DM dogs. The sympathetic activity was suppressed as shown by decrease in both plasma NE concentration and LF component.
采用心率变异性（HRV）和血浆去甲肾上腺素（NE）浓度测量评估患有糖尿病（DM）的犬的心脏自主神经病变。将犬分为 2 组；对照组非 DM 组（n = 13）和糖尿病组（n = 22），根据空腹血浆果糖胺浓度进一步分为血糖控制良好的 DM 组（n = 11）和血糖控制不良的 DM 组（n = 11）。连续记录至少 30 分钟的心电图（ECG）以获得 HRV。结果表明，在血糖控制不良的 DM 亚组中，正常 R-R 间隔的平均值（mean N-N）、所有 5 分钟正常 RR 间隔均值的标准差（SDANN）均低于对照组，而心率较高（P < 0.05）。与血糖控制良好的 DM 亚组相比，NNA、SDNN、SDNN 指数和 pNN50% 明显降低（P < 0.05）。与血糖控制良好的 DM 亚组相比，高频（HF）和总功率明显降低，而低频与高频之比（LF/HF）较高（P < 0.05）。此外，在血糖控制不良的 DM 亚组中，血浆 NE 浓度低于对照组（210 ± 37 对 479 ± 74 pg/ml，P < 0.05）。血浆 NE 与血浆果糖胺浓度之间存在显著负相关。结论是，血糖控制不良的 DM 犬发生心脏自主神经病变。交感神经活性受到抑制，表现为血浆 NE 浓度和 LF 成分均降低。
