Lee Sung Hyen, Lillehoj Hyun S, Jang Seung I, Lee Kyung Woo, Kim Duk Kyung, Lillehoj Erik P, Yancey Robert J, Dominowski Paul J
Animal and Natural Resources Institute, Agricultural Research Service-United States Department of Agriculture, Beltsville, MD 20705, USA.
Avian Dis. 2012 Jun;56(2):402-5. doi: 10.1637/9906-082411-ResNote.1.
The effects against avian coccidiosis of two novel adjuvants, Quil A/cholesterol/dimethyl dioctadecyl ammonium bromide/Carbopol (QCDC) and QCDC/Bay R1005 (R)/cytosine-phosphate-guanosine (CpG) oligodeoxynucleotides (CpG ODN [T]) (QCDCRT) emulsified with profilin, a conserved Eimeria recombinant protein, were determined in broiler chickens. Chickens were subcutaneously immunized with isotonic saline (control group), profilin (P), profilin emulsified with QCDC (P-Q), or profilin with QCDCRT (P-QR) at 2 and 9 days post-hatch and orally challenged with 1.0 x 10(4) sporulated oocysts of Eimeria acervulina (EA) at 7 days postimmunization. All profilin-immunized groups showed increased body weight gain when compared to the control group, and the P-QR group had significantly higher body weight gain than did those of the P and P-Q groups following EA challenge infection. All groups immunized with profilin showed significantly decreased intestinal lesions compared with the control group, with the P-QR group showing the lowest intestinal lesions among the profilin-treated groups. Finally, the P-QR group showed greater CD4+/CD8+ and TCR1+/TCR2+ splenocytes and higher antiprofilin serum antibody titers compared with the P and P-Q (or both) groups following EA challenge infection. These results further suggest that vaccination of chickens with profilin, in combination with the QCDCRT adjuvant, may provide a novel control strategy against EA infection in commercial flocks.
在肉鸡中测定了两种新型佐剂，即与保守的艾美耳球虫重组蛋白肌动蛋白结合蛋白乳化的Quil A/胆固醇/二甲基二十八烷基溴化铵/卡波姆（QCDC）和QCDC/ Bay R1005（R）/胞嘧啶-磷酸-鸟嘌呤（CpG）寡脱氧核苷酸（CpG ODN [T]）（QCDCRT）对禽球虫病的影响。在孵化后第2天和第9天，用等渗盐水（对照组）、肌动蛋白结合蛋白（P）、与QCDC乳化的肌动蛋白结合蛋白（P-Q）或与QCDCRT的肌动蛋白结合蛋白（P-QR）对鸡进行皮下免疫，并在免疫后第7天用1.0×10⁴个堆型艾美耳球虫（EA）的孢子化卵囊进行口服攻毒。与对照组相比，所有用肌动蛋白结合蛋白免疫的组体重增加均有所增加，并且在EA攻毒感染后，P-QR组的体重增加显著高于P组和P-Q组。与对照组相比，所有用肌动蛋白结合蛋白免疫的组肠道病变均显著减少，在经肌动蛋白结合蛋白处理的组中，P-QR组的肠道病变最低。最后，在EA攻毒感染后，与P组和P-Q（或两者）组相比，P-QR组显示出更高的CD4⁺/CD8⁺和TCR1⁺/TCR2⁺脾细胞以及更高的抗肌动蛋白结合蛋白血清抗体滴度。这些结果进一步表明，用肌动蛋白结合蛋白联合QCDCRT佐剂对鸡进行疫苗接种可能为商业鸡群中预防EA感染提供一种新的控制策略。
