Department of Chemical and Pharmaceutical Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, Fujian province, PR China.
Pharm Biol. 2012 Nov;50(11):1423-7. doi: 10.3109/13880209.2012.683115. Epub 2012 Sep 11.
The fruit of the Prunus mume Sieb. et Zucc (Rosaceae) is used as a health food or medicinal material in traditional herb medicine for a long time in Eastern Asian countries.
Our present study investigated the hypouricemic effect of the methanol extract from P. mume fruit (MPMF) in mice with potassium oxonate-induced hyperuremia.
Effect of MPMF (35, 70 and 140 mg/kg, p.o.) administrated for 7 days on the serum, liver, urinary uric acid levels and liver xanthine oxidase (XO) activity were assessed in mice.
Hyperuricemic mice induced by potassium oxonate demonstrated an elevation in serum and liver uric acid levels (11.0 mg/dL and 0.52 mg/g tissue) and a reduction in urinary uric acid levels (49.9 mg/dL). Oral administration of 140 mg/kg MPMF for 7 days reversed the abnormalities in serum, liver and urinary uric acid levels (7.1 mg/dL, 0.37 mg/g tissue and 69.7 mg/dL, respectively). In addition, 70 and 140 mg/kg MPMF (3.1 and 2.9 nmol/min per mg protein) inhibited liver XO activity compared with hyperuricemic mice (3.9 nmol/min per mg protein).
The results indicated that the beneficial hypouricaemic effect of MPMF may be mediated, at least in part, by inhibiting XO activity in the liver. Our study suggests that P. mume and its extracts may have a considerable potential for development as an anti-gout agent for clinical application.
青梅（蔷薇科李属）的果实作为一种药食同源的食品，在东亚国家的传统草药医学中被长期使用。
本研究旨在探讨青梅果实甲醇提取物（MPMF）对氧嗪酸钾诱导高尿酸血症小鼠的降血尿酸作用。
通过连续 7 天灌胃给予 MPMF（35、70 和 140 mg/kg），评估其对小鼠血清、肝脏、尿液中尿酸水平及肝脏黄嘌呤氧化酶（XO）活性的影响。
氧嗪酸钾诱导的高尿酸血症小鼠血清和肝脏尿酸水平（分别为 11.0 mg/dL 和 0.52 mg/g 组织）升高，尿液尿酸水平（49.9 mg/dL）降低。连续 7 天给予 140 mg/kg MPMF 可逆转血清、肝脏和尿液尿酸水平的异常（分别为 7.1 mg/dL、0.37 mg/g 组织和 69.7 mg/dL）。此外，70 和 140 mg/kg MPMF（分别为 3.1 和 2.9 nmol/min/mg 蛋白）可抑制肝脏 XO 活性，与高尿酸血症小鼠相比（3.9 nmol/min/mg 蛋白）。
结果表明，MPMF 的降血尿酸作用可能至少部分是通过抑制肝脏 XO 活性介导的。本研究表明，青梅及其提取物具有作为临床应用抗痛风药物的巨大潜力。
