Department Biology, Marine and Environmental Sciences Faculty, University of Cadiz, Puerto Real, Cadiz, Spain.
Mar Pollut Bull. 2012 Oct;64(10):2097-108. doi: 10.1016/j.marpolbul.2012.07.017. Epub 2012 Aug 2.
The biochemical responses in muscle, such as the enzymatic activities of acetylcholinesterase, lactate dehydrogenase and isocitrate dehydrogenase, were studied in sole (Solea senegalensis) collected in Huelva estuary (SW Spain), in the vicinity of a petrochemical and mining industry. The sampling sites showed different type and degree of pollution. The results demonstrated significant differences in muscle activities of AChE and IDH in Odiel and Tinto Rivers compared to control fish. LDH activity did not show any difference between sampling sites. Significant correlations were established between some biomarkers and heavy metals: AChE was correlated with Pb, Cd and Cu concentrations in water; IDH activity was correlated with Cd and Cu concentrations in water and As, Pb and Cd concentrations in sediments; LDH activity was correlated with As and Zn concentration in water and Cd concentration in sediment. Only one correlation was established between the biomarkers analysed and the concentrations of PAHs: benzo(b)fluoranthene concentration in sediment and IDH.
研究了西班牙韦尔瓦河口（西南西班牙）附近一个石油化工和采矿业附近采集的牙鲆肌肉中的生化反应，如乙酰胆碱酯酶、乳酸脱氢酶和异柠檬酸脱氢酶的酶活性。采样点显示出不同类型和程度的污染。结果表明，与对照鱼相比，在奥德耶和廷托河的肌肉中 AChE 和 IDH 的活性有显著差异。LDH 活性在采样点之间没有差异。一些生物标志物与重金属之间建立了显著的相关性：AChE 与水中的 Pb、Cd 和 Cu 浓度相关；IDH 活性与水中的 Cd 和 Cu 浓度以及沉积物中的 As、Pb 和 Cd 浓度相关；LDH 活性与水中的 As 和 Zn 浓度以及沉积物中的 Cd 浓度相关。在分析的生物标志物与多环芳烃（PAHs）的浓度之间仅建立了一个相关性：沉积物中苯并（b）荧蒽浓度与 IDH。
