Celio M R, Baier W, Schärer L, Gregersen H J, de Viragh P A, Norman A W
Institute of Histology, University of Fribourg, Switzerland.
Cell Calcium. 1990 Oct;11(9):599-602. doi: 10.1016/0143-4160(90)90014-l.
We have produced 25 clones secreting antibodies directed against chicken Calbindin D-28k. Two of them, 300 and 318, recognize determinants conserved in fish, chicken, mouse, rat, rabbit, monkey and human Calbindin D-28k. We demonstrate their use in the immunohistochemical localization of Calbindin D-28k, and in the detection of Calbindin D-28k on immunoblots.
我们已经制备了25个分泌抗鸡钙结合蛋白D-28k抗体的克隆。其中两个克隆,300和318,能识别在鱼类、鸡、小鼠、大鼠、兔子、猴子和人类钙结合蛋白D-28k中保守的决定簇。我们展示了它们在钙结合蛋白D-28k免疫组织化学定位以及免疫印迹法检测钙结合蛋白D-28k中的应用。