Plant Biology and Conservation, Chicago Botanic Garden, Glencoe, Illinois 60022, USA.
Am J Bot. 2012 Aug;99(8):e313-6. doi: 10.3732/ajb.1200003. Epub 2012 Aug 1.
Microsatellite markers were developed in the annual herb, Oenothera harringtonii, to investigate patterns of genetic diversity, gene flow, and parentage within and among populations of this Colorado endemic.
Ten polymorphic loci were identified in O. harringtonii and tested in four populations sampled across the range of the species. These loci contained trinucleotide repeats with 7-29 alleles per locus. Nine of the 10 loci also amplified in O. caespitosa subsp. macroglottis, O. caespitosa subsp. marginata, and O. caespitosa subsp. navajoensis. In addition, we optimized three markers developed for O. biennis and provide reports of their effectiveness in all four taxa.
These results indicate the utility of these markers in O. harringtonii for future studies of genetic structure, gene flow, and parentage as well as their applicability in other members of the O. caespitosa species complex.
本研究在一年生草本植物月见草（Oenothera harringtonii）中开发了微卫星标记，以调查该科罗拉多特有种的种群内和种群间遗传多样性、基因流和亲子关系的模式。
在 O. harringtonii 中鉴定了 10 个多态性位点，并在跨越该物种分布范围的四个种群中进行了测试。这些位点包含三核苷酸重复，每个位点有 7-29 个等位基因。在 O. caespitosa subsp. macroglottis、O. caespitosa subsp. marginata 和 O. caespitosa subsp. navajoensis 中，有 9 个位点也能扩增。此外，我们优化了为 O. biennis 开发的三个标记，并报告了它们在所有四个分类群中的有效性。
这些结果表明，这些标记在 O. harringtonii 中的应用具有未来研究遗传结构、基因流和亲子关系的潜力，也适用于 O. caespitosa 种复合体的其他成员。
