Zhao Li-jun, Men Xiu-li, Kong Xiao-yan, Li Hong-jie, Zhao Xia, Zhang Lian-yuan
Department of Pathophysiology, Hebei United University, Tangshan, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2012 May;28(3):281-3.
To investigate the preventive effects of Salvia miltiorrhiza (SM) on multiple organ edema in the rats which suffered from hind limb ischemia/reperfusion( LI/R).
Twenty four Wistar rats were randomly divided into 3 groups (n = 8): control group (C group), ischemia/reperfusion group (I/R group ), Salvia miltiorrhiza group (SM group). Referring to Tourniquet method, the model rats which underwent 4 hours ischemia and 4 hours reperfusion of hind limbs were made. Thirty minutes before reperfusion, SM was given to the rats in SM group by tail vein injection at the dose of 5 mL/kg. Accurately weighed one gram of heart, liver, kidney, lung, brain, intestine and skeletal muscle from every animals, weigh these specimens after baking (60 degrees C, 55 hours), calculated the ratio of wet and dry (Wet/Dry,W/D). The levels of interleukin-1 (IL-1) ,interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) in plasma and the contents of Superoxide dismutase (SOD) and malonaldehyde (MDA) were measured. The morphologic changes of skeletal muscle were observed with microscope.
It was found that after suffering from ischemia/reperfusion, the W/D of every specimens increased in different degree (P < 0.05, P < 0.01). In plasma, the values of SOD decreased but MDA increased obviously (P < 0.05, P < 0.01). The levels of IL-1, IL-6 ,TNF-alpha-a in plasma increased (P < 0.05, P <0.01). After LI/R, infiltration of inflammatory cells, broaden interstitial around muscle fiber and disordered arrangement of muscle fibers could be seen under microscope. However, Compared with LI/R group, W/D and levels of serum inflammatory factors in SM group were all lower, the values of SOD in plasma increased but MDA in plasma failed down. Pathological changes in skeletal muscle were improved.
Limb ischemia/reperfusion can lead to multiple organ edema, Salvia miltiorrhiza can prevent the edema in some degree by anti-oxidation and anti-inflammation.
探讨丹参对后肢缺血/再灌注（LI/R）大鼠多器官水肿的预防作用。
将24只Wistar大鼠随机分为3组（n = 8）：对照组（C组）、缺血/再灌注组（I/R组）、丹参组（SM组）。参照止血带法制作后肢缺血4小时、再灌注4小时的模型大鼠。再灌注前30分钟，给SM组大鼠尾静脉注射丹参，剂量为5 mL/kg。准确称取每只动物的心脏、肝脏、肾脏、肺、脑、肠和骨骼肌各1克，于60℃烘烤55小时后称重，计算湿干比（Wet/Dry，W/D）。检测血浆中白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）水平以及超氧化物歧化酶（SOD）和丙二醛（MDA）含量。用显微镜观察骨骼肌的形态学变化。
发现缺血/再灌注后，各标本的W/D均有不同程度升高（P < 0.05，P < 0.01）。血浆中SOD值降低而MDA明显升高（P < 0.05，P < 0.01）。血浆中IL-1、IL-6、TNF-α水平升高（P < 0.05，P < 0.01）。LI/R后，显微镜下可见炎性细胞浸润、肌纤维周围间质增宽及肌纤维排列紊乱。然而，与LI/R组相比，SM组的W/D及血清炎性因子水平均较低，血浆中SOD值升高而MDA值下降。骨骼肌病理变化得到改善。
肢体缺血/再灌注可导致多器官水肿，丹参可通过抗氧化和抗炎作用在一定程度上预防水肿。
