[菱角坚果壳中含有的α-葡萄糖苷酶抑制活性成分]

[Alpha-glucosidase inhibitory active constituents contained in nutshell of Trapa acornis].

作者信息

Chen Baiquan, Zhang Qian, Wang Wei, Huang Huan, Kang Wenyi

机构信息

Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2012 May;37(10):1408-11.

PMID:22860451

Abstract

OBJECTIVE

To study chemical constituents in nutshell of Trapa acornis and in vitro inhibitory activity against alpha-glucosidase.

METHOD

EtOAC and n-butanol extractive fractions were separated by chromatography and their structures were identified by multiple spectroscopic techniques.

RESULT

Nine compounds were separated, they were 4,23,24-trimethylcholest-22-en-3-ol (1), stigmasterol (2), alpha-amyrin (3), (+)-nyasol (4), oleanolic acid (5), ursolic acid (6), hederagenin (7), 3,23-dihydroxy-12-ursen-28-oic acid (8) and beta-daucosterol (9). Total extracts from T. acornis nutshells, petroleum ether fractions, acetic ether fractions and normal butanol fractions showed inhibitory activity against alpha-glucosidase. Compound 5 (IC50 2.88 mg x L(-1)) and 6 (IC50 4.42 mg L(-1)) showed stronger inhibitory activity against alpha-glucosidase.

CONCLUSION

All compounds except compound 2 were separated from the genus for the first time, and compound 1-9 were separated from this plant for the first time.

摘要

目的

研究芡实壳中的化学成分及其对α-葡萄糖苷酶的体外抑制活性。

方法

采用柱色谱法分离乙酸乙酯和正丁醇萃取部位，并通过多种波谱技术鉴定其结构。

结果

分离得到9个化合物，分别为4,23,24-三甲基胆甾-22-烯-3-醇（1）、豆甾醇（2）、α-香树脂醇（3）、（+）-尼亚醇（4）、齐墩果酸（5）、熊果酸（6）、常春藤皂苷元（7）、3,23-二羟基-12-乌苏烯-28-酸（8）和β-胡萝卜苷（9）。芡实壳总提取物、石油醚部位、乙酸乙酯部位和正丁醇部位均显示出对α-葡萄糖苷酶的抑制活性。化合物5（IC50 2.88 mg·L-1）和6（IC50 4.42 mg·L-1）对α-葡萄糖苷酶表现出较强的抑制活性。