[斑叶芒毛苣苔的α-葡萄糖苷酶抑制活性]
[Alpha-glucosidase inhibitory activity of Aeschynanthus maculatus].
作者信息
Tian Pu-yu, Kang Wen-yi
机构信息
Institute of Chinese Medica Materia, He'nan University, Kaifeng 475004, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2012 Oct;37(19):2910-2.
OBJECTIVE
To study the inhibitory activity of Aeschynanthus maculatus on alpha-glucosidase.
METHOD
The inhibilitory model of in vitro alpha-glucosidase was established. Active extracts of A. maculatus were isolated and identified bymultiple chromatographic methods, and their molecular structures were identifiied by spectral techniques.
RESULT
Seven coumpounts were isolated from A. maculatus and isolated as lupeol(1), stigmasterol(2), ursolic acid(3), stigmast-5,22(E)-diene-3beta-ol(4), beta-daucosterol(5), 3-hydroxy-12-taraxasten-28-oic-acid(6) and oleanic acid(7). Compounds 1 (IC50 25.41 mg x L(-1)),3(IC0 4.42 mg L(-1)),4(IC50 11.50 mg x L(-1)),6(IC50 14.17 mg x L(-1)) and 7(IC50 2.88 mg x L(-1)) had higher inhibitory activities than that of acarbose (IC50 1103.01 mg x L(-1)) as the positive control drug.
CONCLUSION
Compound 1-7 were isolated from this plant for the first time. Compound 6 was isolated from Gesneriaceae family for the first time. Compound 7 was isolated from Aeschynanthus genus for the first time.
目的
研究红纹秋海棠对α-葡萄糖苷酶的抑制活性。
方法
建立体外α-葡萄糖苷酶抑制模型。采用多种色谱方法对红纹秋海棠的活性提取物进行分离鉴定，并用光谱技术鉴定其分子结构。
结果
从红纹秋海棠中分离得到7个化合物，分别鉴定为羽扇豆醇（1）、豆甾醇（2）、熊果酸（3）、豆甾-5,22（E）-二烯-3β-醇（4）、β-胡萝卜苷（5）、3-羟基-12-蒲公英甾醇-28-酸（6）和齐墩果酸（7）。化合物1（IC50 25.41 mg·L⁻¹）、3（IC50 4.42 mg·L⁻¹）、4（IC50 11.50 mg·L⁻¹）、6（IC50 14.17 mg·L⁻¹）和7（IC50 2.88 mg·L⁻¹）的抑制活性高于作为阳性对照药物的阿卡波糖（IC50 1103.01 mg·L⁻¹）。
结论
化合物1 - 7首次从该植物中分离得到。化合物6首次从苦苣苔科植物中分离得到。化合物7首次从秋海棠属植物中分离得到。
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