Li Jiaxi, Wang Shouchuan, Xu Jianya, Dai Qigang, Xu Shan, Sun Handan, Peng Lulu
Pediatric Institution of Nanjing University of Chinese Medicine, Nanjing 210029, China.
Zhongguo Zhong Yao Za Zhi. 2012 May;37(10):1451-4.
To study the regulation trend of resveratrol on TNF-alpha, IL-1beta, IL-6 expressions in bronchoalveolar layage fluid (BALF) of RSV-infected BALB/c mice at different time points.
RSV-induced BALB/c mice were orally administered with resveratrol. Their BALFs were collected at 24, 72 and 144 h after the first nasal drip with RSV to detect the level of TNF-alpha, IL-1P3, IL-6 by EILSA.
The expression of TNF-alpha, IL-1Pf and IL-6 in BALF increased significantly compared with the normal group (P <0. 01) after 24 hours of RSV infection, while the expression of TNF-alpha (P < 0.01), IL-1beta (P < 0.05), IL-6 (P < 0.01) in the resveratrol group decreased notably compared with the model group. After 72 hours of infection with RSV, although the expression of TNF-alpha (P < 0.05), IL-1beta (P < 0.01) and IL-6 (P < 0.01) in BALF in model group were higher than those in the normal group, they were much more lower than at 24 h. The expression of IL-1beta and IL-6 (P < 0.05) in the resveratrol groups were down-regulated significantly, but no difference had been shown in TNF-alpha expression compared with the RSV infection group. After infection with RSV for 144 h, the expression of IL-1beta (P < 0.01) and IL-6 (P < 0.05) in BALF in the model group were higher than those in the normal group, but there was no difference in the secretion of TNF-alpha. The expression of TNF-alpha, IL-1beta and IL-6 showed also no remarkable difference between the resveratrol groups and the RSV infection group.
Resveratrol can inhibit the over expression of inflammatory factors TNF-alpha, IL-1beta, IL-6 in bronchoalveolar lavage fluid of RSV-induced BALB/c mice and keep them at a low level with the passing of infection time.
研究白藜芦醇对呼吸道合胞病毒（RSV）感染的BALB/c小鼠支气管肺泡灌洗液（BALF）中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）在不同时间点表达的调控趋势。
用RSV诱导BALB/c小鼠，对其口服给予白藜芦醇。在首次经鼻滴注RSV后24、72和144小时收集它们的BALF，通过酶联免疫吸附测定法（ELISA）检测TNF-α、IL-1β、IL-6的水平。
RSV感染24小时后，BALF中TNF-α、IL-1β和IL-6的表达与正常组相比显著增加（P<0.01），而白藜芦醇组中TNF-α（P<0.01）、IL-1β（P<0.05）、IL-6（P<0.01）的表达与模型组相比明显降低。RSV感染72小时后，虽然模型组BALF中TNF-α（P<0.05）、IL-1β（P<0.01）和IL-6（P<0.01）的表达高于正常组，但比24小时时低得多。白藜芦醇组中IL-1β和IL-6（P<0.05）的表达明显下调，但与RSV感染组相比，TNF-α表达无差异。RSV感染144小时后，模型组BALF中IL-1β（P<0.01）和IL-6（P<0.05）的表达高于正常组，但TNF-α分泌无差异。白藜芦醇组与RSV感染组之间TNF-α、IL-1β和IL-6的表达也无显著差异。
白藜芦醇可抑制RSV诱导的BALB/c小鼠支气管肺泡灌洗液中炎症因子TNF-α、IL-1β、IL-6的过度表达，并随着感染时间的推移使其保持在低水平。
