Cheng Kebin, Wu Zhenghua, Gao Beilan, Xu Jinfu
Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, 200433, China.
Cell Biochem Biophys. 2014 Nov;70(2):1305-9. doi: 10.1007/s12013-014-0055-9.
Explore the influence of baicalin joint resveratrol retention enema on TNF-α, SIgA, IL-2, and IFN-γ of rats with respiratory syncytial virus (RSV) infection. The 60 SD rats were randomly divided into normal group, model group, baicalin group, resveratrol group, joint group, and ribavirin group. For model group, baicalin group, resveratrol group, joint group, and ribavirin group, rats were given RSV virus suspension intranasally for 3 days, and model group was not given administration. Baicalin group, resveratrol group, joint group, and ribavirin group were, respectively, given baicalin 100 mg/kg/day, resveratrol 30 mg/kg/day, baicalin joint resveratrol, and ribavirin 1 g/kg/day retention enema. After continuously given administration 7 days, rats were measured in serum TNF-α, IL-2, IFN-γ levels and SIgA levels in bronchoalveolar lavage fluid. Model group, TNF-α, IL-2, IFN-γ, and SIgA were significantly higher than the normal group (P < 0.05); Baicalin group, resveratrol group, ribavirin group, TNF-α, IL-2, IFN-γ, and SIgA were significantly higher than the model group (P < 0.05); TNF-α, IL-2 between baicalin group, resveratrol group, ribavirin group, have no significant difference (P > 0.05); Baicalin group, resveratrol group, joint group, IFN-γ, and SIgA were significantly higher than the ribavirin group (P < 0.05); Joint group TNF-α, IL-2, IFN-γ, and SIgA were significantly higher than baicalin group, resveratrol group, and ribavirin group (P < 0.05). Baicalin joint resveratrol retention enema can increase RSV infection model in rats serum TNF-α, IL-2, IFN-γ levels and SIgA levels in bronchoalveolar lavage fluid, which may anti-virus through this mechanism.
探讨黄芩苷联合白藜芦醇保留灌肠对呼吸道合胞病毒（RSV）感染大鼠肿瘤坏死因子-α（TNF-α）、分泌型免疫球蛋白A（SIgA）、白细胞介素-2（IL-2）及干扰素-γ（IFN-γ）的影响。将60只SD大鼠随机分为正常组、模型组、黄芩苷组、白藜芦醇组、联合组及利巴韦林组。对模型组、黄芩苷组、白藜芦醇组、联合组及利巴韦林组大鼠经鼻给予RSV病毒悬液3天，正常组不给予处理。黄芩苷组、白藜芦醇组、联合组及利巴韦林组分别给予黄芩苷100mg/kg/天、白藜芦醇30mg/kg/天、黄芩苷联合白藜芦醇及利巴韦林1g/kg/天保留灌肠。连续给药7天后，检测大鼠血清中TNF-α、IL-2、IFN-γ水平及支气管肺泡灌洗液中SIgA水平。模型组TNF-α、IL-2、IFN-γ及SIgA水平显著高于正常组（P<0.05）；黄芩苷组、白藜芦醇组、利巴韦林组TNF-α、IL-2、IFN-γ及SIgA水平显著高于模型组（P<0.05）；黄芩苷组、白藜芦醇组、利巴韦林组之间TNF-α、IL-2比较差异无统计学意义（P>0.05）；黄芩苷组、白藜芦醇组、联合组IFN-γ及SIgA水平显著高于利巴韦林组（P<0.05）；联合组TNF-α、IL-2、IFN-γ及SIgA水平显著高于黄芩苷组、白藜芦醇组及利巴韦林组（P<0.05）。黄芩苷联合白藜芦醇保留灌肠可提高RSV感染模型大鼠血清TNF-α、IL-2、IFN-γ水平及支气管肺泡灌洗液中SIgA水平，其可能通过此机制发挥抗病毒作用。
