Liang Xinli, Zhu Jingyun, Liao Zhenggen, Zhao Lijun, Zhao Guowei, Yang Ming, Cao Yunchao
Key Laboratory of Modern Preparation of Traditional Chinese medicine, Jiangxi University of Traditional Chinese Medicine, Ministry of Education, Nanchang 330004, China.
Zhongguo Zhong Yao Za Zhi. 2012 May;37(10):1477-82.
To study the effect of extractive Angelica dahutica on intestinal absorption of puerarin, the mechanism of the absorption enhancement of A. dahutica was investigated, providing a new thread of combinations of the Chinese herbal drugs.
Everted intestinal sac and in situ single pass perfusion were used to study the effect of gut absorption of puerarin solution containing the extractive A. dahurica as well as the influence of P-gp on the absorption of puerarin, and explore weather the extractive A. dahutica can enhance the absorption of puerarin and the mechanism of absorption of puerarin.
The puerarin could be absorbed at all of four intestinal segments with increaing absorption amount perunit as follows: ileum > colon > jejunum > duodenum. The absorption of puerarin in jejunum was significantly increased with the extractive A. dahutica in situ single pass perfusion of jejunum. The apparent permeability coefficient (Papp) and absorption rate constant (Ka) of puerarin in the jejunum were descreased gradually with higher concentrations, and the Papp with the Ka of jejunum solution containing the P-gp inhibitor of verapamil were increasing respectively 2.49, 2.60 (P < 0.001) than only the jejunum solution in absorption. The absorption of jejunum in pH 5.0, 6.8 were better than it in pH 7.4.
The mechanism of absorption of jejunum was active absorption and was effected by P-gp. The extrative A. dahurica can enhance the absorption of the jejunum.
研究白芷提取物对葛根素肠道吸收的影响，探讨白芷促吸收作用机制，为中药配伍提供新思路。
采用外翻肠囊法和原位单向灌流法，研究含白芷提取物的葛根素溶液的肠道吸收情况以及P-糖蛋白对葛根素吸收的影响，探讨白芷提取物能否促进葛根素的吸收及其吸收机制。
葛根素在肠道4个肠段均有吸收，单位吸收量大小顺序为：回肠＞结肠＞空肠＞十二指肠。原位单向灌流空肠实验表明，含白芷提取物可显著增加葛根素在空肠的吸收。葛根素空肠吸收的表观渗透系数（Papp）和吸收速率常数（Ka）随浓度升高逐渐降低，加入P-糖蛋白抑制剂维拉帕米后，空肠溶液的Papp和Ka分别比单纯空肠溶液吸收时增加2.49、2.60（P＜0.001）。葛根素在pH 5.0、6.8时的空肠吸收情况优于pH 7.4时。
葛根素空肠吸收机制为主动转运，且受P-糖蛋白影响。白芷提取物能促进葛根素在空肠的吸收。
