Zhu Jing-yun, Liang Xin-li, Wang Guang-fa, Zhao Guo-wei, Liao Zheng-gen, Cao Yun-chao, Chen Xu-long, Yang Ming
Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Yao Xue Xue Bao. 2011 Feb;46(2):232-7.
To explore the mechanism of the absorption enhancement of Angelica dahurica extract (Ade), the absorption mechanism of baicalin in the Scutcllaria water extraction as well as the effect of Angelica dahurica extract on absorption of baicalin were investigated. In order to determine the main absorption site, everted intestinal sac model was used to study the effect of Angelica dahurica extract on the absorption of baicalin at duodenum, jejunum, ileum and colon. In situ single pass intestinal perfusion model was performed to study the absorption of various concentrations of baicalin and the effect of Angelica dahurica extract on the absorption of baicalin at the main absorption site. To authenticate the consequence of perfusion by getting the blood from the hepatic portal vein and determine the concentration of the baicalin in the blood. The result showed that baicalin could be absorbed at all of the four intestinal segments with increasing absorption amount per unit as follows: ileum > colon > jejunum > duodenum. The absorption ofbaicalin in the duodenum significantly increased with Angelica dahurica extract, thus, duodenum was chosen to be the studying site. Apparent permeability values (Papp) and absorption rate constant (Ka) of baicalin in the duodenum increased gradually with higher concentrations. When the concentration of baicalin rises to a certain degree, the absorption increase had a saturable process, the absorption of baicalin may be an active transportation. Baicalin may be not a substrate of P-gp as verapamil which had not significantly affected the Papp and Ka of baicalin. The absorption of baicalin in the duodenum significantly increased (P < 0.01) in the two models with Angelica dahurica extract and the concentration of baicalin in the blood from the hepatic portal vein showed that the Angelica dahurica extract can increase the absorption of baicalin.
为探讨白芷提取物（Ade）吸收促进的机制，研究了黄芩水提取物中黄芩苷的吸收机制以及白芷提取物对黄芩苷吸收的影响。为确定主要吸收部位，采用外翻肠囊模型研究白芷提取物对黄芩苷在十二指肠、空肠、回肠和结肠吸收的影响。采用原位单向肠灌注模型研究不同浓度黄芩苷的吸收以及白芷提取物对主要吸收部位黄芩苷吸收的影响。通过从肝门静脉取血来验证灌注结果并测定血液中黄芩苷的浓度。结果表明，黄芩苷在所有四个肠段均可吸收，单位吸收量增加顺序为：回肠＞结肠＞空肠＞十二指肠。黄芩苷在十二指肠的吸收随白芷提取物显著增加，因此，选择十二指肠作为研究部位。黄芩苷在十二指肠的表观渗透系数（Papp）和吸收速率常数（Ka）随浓度升高而逐渐增加。当黄芩苷浓度升高到一定程度时，吸收增加有饱和过程，黄芩苷的吸收可能是主动转运。黄芩苷可能不是P-糖蛋白的底物，因为维拉帕米对黄芩苷的Papp和Ka没有显著影响。在两个模型中，白芷提取物显著增加了黄芩苷在十二指肠的吸收（P＜0.01），且肝门静脉血中黄芩苷的浓度表明白芷提取物可增加黄芩苷的吸收。
