Delezuch W, Marttinen P, Kokki H, Heikkinen M, Vanamo K, Pulkki K, Matinlauri I
Department of Clinical Chemistry, University of Kuopio and Eastern Finland Laboratory Centre, Kuopio, Finland.
Tissue Antigens. 2012 Oct;80(4):368-75. doi: 10.1111/j.1399-0039.2012.01938.x. Epub 2012 Aug 3.
Activated T-helper type 1 (Th1) lymphocytes induce a cellular type immune response, and Th2 lymphocytes, a humoral or antibody-mediated type immune response. Soluble CD26 (sCD26) and soluble CD30 (sCD30) are regarded as markers of Th1 and Th2 lymphocyte activation, respectively. Serum from 112 generally healthy pediatric surgical patients and cerebrospinal fluid (CSF) from 39, aged 1-17 years were measured for sCD26 and sCD30 using an enzyme-linked immunosorbent assay method. The detection limit for sCD26 was 6.8 ng/ml and for sCD30, 1.9 IU/ml. For serum sCD26 and sCD30, 2.5% and 97.5% percentiles constituted the reference limits, and the 95% credible intervals for the percentiles were calculated using regression models with a Bayesian approach. A significant between-gender difference was observed (P = 0.015) in serum sCD26 concentration, of which the lower limits ranged between 273 and 716 ng/ml for girls and 235 and 797 ng/ml for boys. The upper limits ranged between 1456 and 1898 ng/ml for girls and between 1419 and 1981 ng/ml for boys. Moreover, the concentrations of sCD26 increased in infants and children up to 10 years in girls and 12 years in boys. After this however, the values decreased. The serum sCD30 concentration was highest among the youngest infants aged 1 year (80-193 IU/ml), after which a consistent age-related decrease was found. The lowest values were found at the age of 17 years (10-89 IU/ml). A significant between-gender difference in sCD30 concentration was observed (P = 0.019). sCD26 and sCD30 concentrations were low in the CSF samples analyzed: 13.3 ng/ml (median); range 8.3-51.5 ng/ml and 7.6 IU/ml; 2.1-18.5 IU/ml, respectively. Reference limits for serum sCD26 in children aged 1-17 years were established as being 235-1800 ng/ml in toddlers and 400-1800 ng/ml in female adolescents and 700-2000 ng/ml in male adolescents. For sCD30; reference limits of 80-190 IU/ml were established in the youngest age group and 10-90 IU/ml in adolescents.
活化的1型辅助性T（Th1）淋巴细胞诱导细胞免疫反应，而Th2淋巴细胞则诱导体液或抗体介导的免疫反应。可溶性CD26（sCD26）和可溶性CD30（sCD30）分别被视为Th1和Th2淋巴细胞活化的标志物。采用酶联免疫吸附测定法对112名一般健康的儿科手术患者的血清以及39名年龄在1至17岁之间患者的脑脊液（CSF）进行了sCD26和sCD30检测。sCD26的检测限为6.8 ng/ml，sCD30的检测限为1.9 IU/ml。对于血清sCD26和sCD30，以第2.5百分位数和第97.5百分位数作为参考限值，并使用贝叶斯方法的回归模型计算这些百分位数的95%可信区间。观察到血清sCD26浓度存在显著的性别差异（P = 0.015），女孩的下限范围为273至716 ng/ml，男孩为235至797 ng/ml。女孩的上限范围为1456至1898 ng/ml，男孩为1419至1981 ng/ml。此外，女孩中10岁及以下、男孩中12岁及以下婴幼儿和儿童的sCD26浓度升高。然而在此之后，数值下降。血清sCD30浓度在1岁的最年幼婴儿中最高（80 - 193 IU/ml），之后发现其随年龄持续下降。在17岁时发现最低值（10 - 89 IU/ml）。观察到sCD30浓度存在显著的性别差异（P = 0.019）。所分析的脑脊液样本中sCD26和sCD30浓度较低：中位数分别为13.3 ng/ml；范围为8.3 - 51.5 ng/ml以及7.6 IU/ml；2.1 - 18.5 IU/ml。1至17岁儿童血清sCD26的参考限值确定为：幼儿为235 - 1800 ng/ml，女性青少年为400 - 1800 ng/ml，男性青少年为700 - 2000 ng/ml。对于sCD30；最年幼年龄组的参考限值为80 - 190 IU/ml，青少年为10 - 90 IU/ml。
