From the Department of Health Informatics and Technologies, Faculty of Health Sciences Department of Endodontics, Faculty of Dentistry, Marmara University, Istanbul Department of Oral and Maxillofacial Surgery, Gulhane School of Medicine, Ankara Department of Rheumatology Institute of GastroenterologyDepartments of Haematology-Immunology Dermatology, Faculty of Medicine, Marmara University, Istanbul, Turkey.
Int J Dermatol. 2013 Oct;52(10):1198-201. doi: 10.1111/j.1365-4632.2012.05504.x. Epub 2012 Aug 2.
Saliva contains antimicrobial peptides derived from oral epithelium as well as neutrophils in the innate immune response. The aim of this study was to examine the association between salivary human neutrophil peptide (HNP) 1-3 levels originating from neutrophils and oral ulcers in patients with Behçet's disease (BD).
Ninety-five patients with BD (F/M: 39/56; mean age: 38.7 ± 11.9 years) and 53 healthy controls (HC; F/M: 23/30; mean age: 35.2 ± 10.1 years) were included in the study. The disease control group (F/M: 20/33; mean age: 33.7 ± 10.7 years) was comprised of patients with oral infection regarding endodontic infection (n = 32) and pericoronitis (n = 21). Salivary HNP 1-3 levels of groups were measured in unstimulated samples by ELISA (Hycult, the Netherlands).
A statistically significant increase was found in salivary HNP 1-3 levels of patients with BD (2268.28 ± 1216.38 μg/ml) compared with HC (1836.49 ± 857.76 μg/ml), patients with endodontic infection (849.9 ± 376.1 μg/ml), and patients with pericoronitis (824.3 ± 284.02 μg/ml; P = 0.024, 0.000 and 0.000, respectively). The ratio of active oral ulcer (100%, n = 14) was higher in low HNP 1-3 levels (≤ 1000 μg/ml) than the others (66.7%, n = 54) in active patients with BD (P = 0.008). Moreover, salivary HNP 1-3 levels were significantly lower in patients with endodontic infection and patients with pericoronitis compared with those in the HC group and patients with BD (P = 0.000).
A decrease in salivary HNP 1-3 levels might be a biological factor for predisposition to oral ulcers in patients with BD and oral infection in healthy patients.
唾液中含有来源于口腔上皮细胞和中性粒细胞的抗菌肽。本研究旨在探讨唾液中人中性粒细胞肽（HNP）1-3 水平与白塞病（BD）患者口腔溃疡之间的关系。
纳入 95 例 BD 患者（男/女：39/56；平均年龄：38.7±11.9 岁）和 53 例健康对照者（男/女：23/30；平均年龄：35.2±10.1 岁）。疾病对照组（男/女：20/33；平均年龄：33.7±10.7 岁）由牙髓感染（n=32）和冠周炎（n=21）患者组成。采用 ELISA（荷兰 Hycult 公司）检测各组患者非刺激唾液中 HNP 1-3 的水平。
BD 患者唾液 HNP 1-3 水平（2268.28±1216.38μg/ml）显著高于健康对照者（1836.49±857.76μg/ml）、牙髓感染患者（849.9±376.1μg/ml）和冠周炎患者（824.3±284.02μg/ml；P=0.024、0.000 和 0.000）。BD 活动期患者中，低 HNP 1-3 水平（≤1000μg/ml）组的活动性口腔溃疡比例（100%，n=14）高于其他组（66.7%，n=54）（P=0.008）。此外，与健康对照组和 BD 患者相比，牙髓感染和冠周炎患者的唾液 HNP 1-3 水平显著降低（P=0.000）。
唾液 HNP 1-3 水平降低可能是 BD 患者和健康患者口腔感染发生口腔溃疡的易感性的生物学因素。
