Department of Health Management, Marmara University, Istanbul, Turkey. goncamumcu.edu.tr
Clin Exp Rheumatol. 2009 Mar-Apr;27(2 Suppl 53):S32-6.
The present study aimed to investigate the interactions among salivary S. mutans colonisation, serum mannose binding lectin level (MBL), oral ulcer activity and disease course in patients with Behçet's disease (BD).
One hundred and six BD patients, 25 patients with rheumatoid arthritis (RA) and 42 healthy controls (HC) were included in the study. BD patients were grouped as active (n=52) or inactive (n=54) according to oral ulcer status of the previous 3 months. Salivary colonisation of S. mutans levels were investigated by standard Caries Risk Test (CRT) Bacteria kits (Ivoclar, Vivadent). S. mutans colonies were categorized as high (> or =10(5) colony forming unit (CFU)/ml of saliva) or low (10(5)CFU/ml). Serum mannose binding lectin (MBL) levels were measured by ELISA.
High levels of salivary S. mutans colonisation was significantly more present in BD (50%) than HC (28.6%)(p=0.039), whereas no significant difference was observed between RA and other groups (p>0.05). S. mutans presence in saliva was associated with oral ulcers (61.5% in patients with active oral ulcers vs 38.9% in inactives) (p=0.020). S. mutans colonisation in saliva was significantly higher among male BD patients with a severe disease course than a milder disease (p=0.04). Increased salivary S. mutans colonisation was also related to very low serum MBL (<100 ng/ml) in BD compared to controls (p=0.04).
The relationship between increased presence of S. mutans and MBL deficiency with active disease pattern may indicate an impaired innate immune response in BD patients which may predispose to oral infections and a severe disease course.
本研究旨在探讨唾液变形链球菌定植、血清甘露糖结合凝集素水平（MBL）、口腔溃疡活动度和贝赫切特病（BD）病程之间的相互关系。
纳入 106 例 BD 患者、25 例类风湿关节炎（RA）患者和 42 名健康对照者（HC）。根据过去 3 个月的口腔溃疡情况，BD 患者分为活动期（n=52）和非活动期（n=54）。采用标准龋病风险测试（CRT）细菌试剂盒（Ivoclar，Vivadent）检测唾液变形链球菌定植水平。变形链球菌菌落分为高（≥10^5菌落形成单位（CFU）/ml 唾液）或低（10^5 CFU/ml）。采用 ELISA 法测定血清甘露糖结合凝集素（MBL）水平。
BD 患者唾液变形链球菌定植水平较高（50%）显著高于 HC（28.6%）（p=0.039），而 RA 与其他组之间无显著差异（p>0.05）。唾液中存在变形链球菌与口腔溃疡有关（活动期口腔溃疡患者中为 61.5%，非活动期为 38.9%）（p=0.020）。唾液中变形链球菌定植水平在男性 BD 患者中较高，且疾病病程较重，而非较轻（p=0.04）。BD 患者唾液中变形链球菌定植水平较高与 MBL 缺乏（<100ng/ml）也有关，与对照者相比，差异有统计学意义（p=0.04）。
变形链球菌定植增加和 MBL 缺乏与活动期疾病模式之间的关系可能表明 BD 患者固有免疫反应受损，这可能使患者易发生口腔感染和疾病严重程度增加。
