EEA La Consulta INTA, IBAM, Universidad Nacional de Cuyo and CONICET, Alte. Brown 500 (M5528AHB), Mendoza, Argentina.
Genome. 2012 Aug;55(8):605-14. doi: 10.1139/g2012-047. Epub 2012 Aug 3.
To investigate the molecular bases of postzygotic hybridization barriers in tuber-bearing Solanums, the wild species Solanum commersonii Dunal ex Poir. (cmm, 2n = 2x = 24, 1EBN) and Solanum acaule Bitter (acl, 2n = 4x = 48, 2EBN) were crossed in intra- and interspecific genotypic combinations, and the transcriptome of immature seeds was analyzed by using the cDNA-AFLP technique. From a total of 423 analyzed cDNA fragments, 107 (25.3%) were differentially regulated in the compatible (acl × acl and cmm × cmm) versus incompatible (acl × cmm) crosses. DNA sequence data were obtained from 21 fragments and RT-PCR analyses were carried out with five fragments to validate the cDNA-AFLP differential pattern. Sequence analysis suggested a possible role for the differentially expressed sequences in cytokinesis, cell cycle, secondary and hormonal metabolism, biodegradation, and transport. In situ hybridization experiments with fragments encoding an ubiquitin-fold modifier 1 precursor and a possible vesicle transport protein revealed expression of these genes in the embryo and endosperm. The results suggest that the collapse of the embryo and endosperm in incompatible crosses may be related to alterations in cell cycle and cytokinesis.
为了研究产薯茄属植物合子后杂交障碍的分子基础，我们对野生种茄属 Solanum commersonii Dunal ex Poir.（cmm，2n = 2x = 24，1EBN）和 Solanum acaule Bitter（acl，2n = 4x = 48，2EBN）进行了种内和种间基因型杂交，并利用 cDNA-AFLP 技术分析了未成熟种子的转录组。在总共分析的 423 个 cDNA 片段中，107 个（25.3%）在亲和（acl×acl 和 cmm×cmm）与不亲和（acl×cmm）杂交中差异表达。从 21 个片段中获得了 DNA 序列数据，并对 5 个片段进行了 RT-PCR 分析，以验证 cDNA-AFLP 差异模式。序列分析表明，差异表达序列可能在细胞分裂、细胞周期、次生和激素代谢、生物降解和运输中发挥作用。与编码泛素折叠修饰物 1 前体和可能的囊泡运输蛋白的片段进行原位杂交实验，显示这些基因在胚胎和胚乳中的表达。结果表明，不亲和杂交中胚胎和胚乳的崩溃可能与细胞周期和细胞分裂的改变有关。
