Department of Pathology Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan.
Atherosclerosis. 2012 Oct;224(2):511-4. doi: 10.1016/j.atherosclerosis.2012.07.027. Epub 2012 Jul 25.
To examine the presence of CD34-positive cells and intranuclear factors in acute coronary thrombi, we compared thrombi in patients with type 2 diabetes mellitus (DM, n = 21) and without DM (n = 29). Immunohistochemical staining revealed the constitutive presence of platelets, fibrin, erythrocytes, neutrophils, extracellular high-mobility group box 1 (HMGB-1), and histone H3 in all thrombi. There were significantly more oval or flat CD34-positive cells and significantly larger HMGB-1-positive areas in the thrombi from patients with DM. The flat CD34-positive cells expressed ecto-nucleoside triphosphate diphosphohydrolase (a platelet aggregation inhibitor). The number of CD34-positive cells was negatively correlated with the serum levels of glucose and hemoglobin A1c, whereas the HMGB-1-positive area was positively correlated with the levels of serum glucose. The paucity of CD34-positive cells and the high levels of HMGB-1 expression in acute coronary thrombi from patients with type 2 DM could facilitate thrombus formation.
为了研究 CD34 阳性细胞和核内因子在急性冠状动脉血栓中的存在情况，我们比较了 21 例 2 型糖尿病（DM）患者和 29 例无 DM 患者的血栓。免疫组织化学染色显示所有血栓中均存在血小板、纤维蛋白、红细胞、中性粒细胞、细胞外高迁移率族蛋白 B1（HMGB-1）和组蛋白 H3。DM 患者血栓中的椭圆形或扁平 CD34 阳性细胞明显更多，HMGB-1 阳性面积明显更大。扁平 CD34 阳性细胞表达外核苷酸三磷酸二磷酸水解酶（血小板聚集抑制剂）。CD34 阳性细胞数量与血清葡萄糖和血红蛋白 A1c 水平呈负相关，而 HMGB-1 阳性面积与血清葡萄糖水平呈正相关。2 型糖尿病患者急性冠状动脉血栓中 CD34 阳性细胞数量减少和 HMGB-1 表达水平升高可能促进血栓形成。
