Papakonstantinou Maritsa K, Pan Wei-Ren, le Roux Cara Michelle, Richardson Martin D
Jack Brockhoff Reconstructive Plastic Surgery Research Unit, Department of Anatomy and Cell Biology, The University of Melbourne, Melbourne, Victoria, Australia.
ANZ J Surg. 2012 Oct;82(10):704-7. doi: 10.1111/j.1445-2197.2012.06142.x. Epub 2012 Aug 2.
The study of intraosseous vasculature has always been challenging due to the hard, calcified structure of bone. Techniques used to study intraosseous vasculature usually involve diaphanization with a Spalteholz technique, followed by X-ray imaging to display the radio contrast-injected blood vessels. These techniques produce results with fine detail when successfully executed. However, high failure rates and the extensive length of time required to perform these techniques are drawbacks. This paper describes a faster, alternative method for the study of intraosseous vasculature.
Five embalmed and two fresh shoulder girdles were harvested from six cadavers. Cannulas were inserted into the origins of the anterior (ACHA) and posterior (PCHA) circumflex humeral arteries and injected with ink diluted in water or 6% hydrogen peroxide. The arteries and their branches were dissected until they entered their respective bony foraminae. A hammer, chisel, bone nibbler and mounted needles were used to follow the intraosseous course of these arteries and their branches.
The intraosseous vasculature was seen in all specimens. The branches of the main nutrient artery to the proximal humerus were followed until they reached articular cartilage or crossed cortical bone again to enter the rotator cuff tendons.
An innovative, new approach to the study of intraosseous vasculature with direct visualization of the intraosseous arteries of the proximal humerus is described.
由于骨骼坚硬、钙化的结构，骨内血管系统的研究一直具有挑战性。用于研究骨内血管系统的技术通常包括使用施帕尔托兹技术进行透明化处理，然后进行X射线成像以显示注入放射性造影剂的血管。这些技术成功执行时能产生细节清晰的结果。然而，这些技术的高失败率以及执行所需的较长时间是其缺点。本文描述了一种更快的、用于研究骨内血管系统的替代方法。
从六具尸体上获取了五个防腐处理的和两个新鲜的肩胛带。将插管插入旋肱前动脉（ACHA）和旋肱后动脉（PCHA）的起始处，并注入用水或6%过氧化氢稀释的墨水。解剖动脉及其分支，直到它们进入各自的骨孔。使用锤子、凿子、咬骨钳和固定针追踪这些动脉及其分支在骨内的行程。
在所有标本中均可见骨内血管系统。追踪了肱骨近端主要滋养动脉的分支，直到它们到达关节软骨或再次穿过皮质骨进入肩袖肌腱。
描述了一种创新的、用于研究骨内血管系统的新方法，可直接观察肱骨近端的骨内动脉。
