Qiu Xing, Shi Xiaotian, Ouyang Jun, Xu Dachuan, Zhao Dewei
Department of Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, School of Basic Medicine Science, Southern Medical University, Guangzhou, Guangdong, China.
Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning, China.
J Anat. 2016 Aug;229(2):326-33. doi: 10.1111/joa.12475. Epub 2016 Apr 14.
We describe a technique for perfusing a barium sulphate suspension into the intraosseous artery. Following the perfusion of abarium sulphate suspension into 14 fresh lower limbs of Chinese cadavers, micro-CT scanning was applied to digitize, quantify and visualize the intraosseous arteries in the human femoral heads. Then, the femoral heads were removed and subjected to micro-CT scanning. The data were imported into the amira and mimics programs to reconstruct and quantify the intraosseous arteries. The femoral head intraosseous artery lengths, areas, volumes, and femoral head bone volumes were quantified. The artery densities and artery ratios were calculated and analysed with independent-samples t-tests. The intraosseous vasculature volume renderings were displayed as screenshots and videos made with amira. Many intraosseous artery study technologies were compared. The barium sulphate suspension was milky white in colour. The perfusion of the barium sulphate suspension followed by micro-CT scanning provided a good representation of the intraosseous artery. The femoral head intraosseous artery lengths, areas and volumes, and the femoral head bone volumes were displayed as the X¯±S . No differences were observed between the left and right femoral head intraosseous arteries in terms of the artery densities or artery ratios. The volume renderings and 3-D orthogonal projections displayed the overall distributions of the intraosseous arteries. The videos clearly demonstrated the entry sites of the nutrition-carrying arteries, their courses and branches, and the intraosseous arterial anastomoses. Our technique is the simplest and least time-consuming method of producing accurate vascular three-dimensional reconstructions. The perfusion of a barium sulphate suspension into intraosseous arteries combined with micro-CT scanning can deliver high-resolution 3-D digitized data and images of intraosseous arteries. This technique does not require bone decalcification or bone dissection and thus significantly shortens the time required to quantify and display intraosseous arteries. This method provides a simple and rapid technique for quantifying and visualizing human intraosseous arteries.
我们描述了一种将硫酸钡混悬液灌注到骨内动脉的技术。将硫酸钡混悬液灌注到14具中国新鲜尸体的下肢后，应用显微CT扫描对人股骨头内的骨内动脉进行数字化、定量分析和可视化处理。然后，将股骨头取出并进行显微CT扫描。数据导入Amira和Mimics程序以重建和定量骨内动脉。对股骨头骨内动脉长度、面积、体积以及股骨头骨体积进行了定量分析。计算动脉密度和动脉比率，并采用独立样本t检验进行分析。骨内血管系统的容积渲染图以Amira制作的截图和视频形式展示。比较了多种骨内动脉研究技术。硫酸钡混悬液呈乳白色。硫酸钡混悬液灌注后进行显微CT扫描能够很好地呈现骨内动脉情况。股骨头骨内动脉长度、面积和体积以及股骨头骨体积以X¯±S表示。左右股骨头骨内动脉在动脉密度或动脉比率方面未观察到差异。容积渲染图和三维正交投影展示了骨内动脉的整体分布情况。视频清晰地显示了营养动脉的进入部位、走行和分支以及骨内动脉吻合情况。我们的技术是生成准确血管三维重建的最简单且最省时的方法。将硫酸钡混悬液灌注到骨内动脉并结合显微CT扫描能够提供高分辨率的骨内动脉三维数字化数据和图像。该技术无需进行骨脱钙或骨解剖，因此显著缩短了定量和显示骨内动脉所需的时间。此方法为定量和可视化人体骨内动脉提供了一种简单快速的技术。
