Tian Mei-juan, Li Yu-yao, Luo Chang-qin, Lv Mei-ling, Yang Jin
Department of Clinical Oncology, Xi'an Jiaotong University, Xi'an, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Aug;28(8):851-5.
To investigate the expression of the oxidative stress proteins in human clear-cell renal cell carcinoma (CRCC) cell line (RLC-310) and normal renal proximal tubule epithelial cell line (HK-2), the CRCC and the corresponding normal renal tissues.
RLC-310 and HK-2 cells were cultured in vitro. Total proteins of the two cell lines were separated by PF-2D protein fractionation system. The differentially expressed proteins of the two cell lines were analyzed using capillary LC-ESI-MS/MS and identified using the protein database. The representative differential oxidative stress proteins were verified by immunohistochemistry in the CRCC and corresponding normal renal tissues.
Twelve differentially expressed oxidative stress proteins were identified, including peroxiredoxin-1, peroxiredoxin-6 (PRX-6), superoxide dismutase[Cu-Zn] SOD1, catalase, glutathione peroxidase 1, glutathione synthetase, glutathione S-transferase Pi (GSTPi), thioredoxin, heat shock protein 10 (HSP10), HSP60, HSP70 and HSP90. Three representative differential proteins PRX-6, HSP60 and GSTPi were both expressed in RLC-310 and HK-2, and the levels of these proteins were significantly higher in RLC-310 than those in HK-2 (P<0.05). The levels of these proteins were significantly higher in CRCC than those in corresponding normal renal tissues (P<0.05).
A series of oxidative stress proteins are overexpressed in the CRCC. They play an important role in preventing oxidative damage of CRCC cells.
