Department of Urology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
J Urol. 2012 Apr;187(4):1172-6. doi: 10.1016/j.juro.2011.11.105. Epub 2012 Feb 14.
Although oxidative stress is implicated in renal cell carcinoma pathogenesis, to our knowledge changes in oxidative stress parameters in patients who undergo surgery for renal cell carcinoma have not been studied previously. We investigated the status of oxidative stress in patients with renal cell carcinoma.
Reactive oxygen species, nitric oxide and glutathione were measured in the blood of 68 patients with renal tumor and in 30 age matched normal controls. Levels were measured again 1 week, and 1 and 2 months postoperatively in patients who underwent surgery for renal cell carcinoma. Levels of superoxide dismutase, catalase and lipid peroxidation were measured in tumor tissue and in normal renal parenchyma in 51 patients with renal tumor.
Significantly increased reactive oxygen species and nitric oxide, and decreased glutathione were observed in patients with renal cell carcinoma compared to normal subjects and in patients with benign tumors. Superoxide dismutase and lipid peroxidation were increased and catalase was decreased in tumor tissue compared to normal renal tissue. Oxidative stress correlated with renal cell carcinoma grade and stage but decreased after curative resection. Patients with metastatic disease had persistently increased oxidative stress parameters. Antioxidant enzyme levels in benign tumor tissue were significantly higher than in renal cell carcinoma.
Patients with renal cell carcinoma have increased oxidative stress, which is effectively alleviated by curative resection. In patients with benign tumors antioxidant defense mechanisms maintain normal redox status.
尽管氧化应激与肾细胞癌的发病机制有关，但据我们所知，以前尚未研究过接受肾细胞癌手术的患者的氧化应激参数变化。我们研究了肾细胞癌患者的氧化应激状态。
测量了 68 例肾肿瘤患者和 30 例年龄匹配的正常对照者的血液中活性氧、一氧化氮和谷胱甘肽的水平。在接受肾细胞癌手术的患者中，分别于术后 1 周、1 个月和 2 个月再次测量这些水平。在 51 例肾肿瘤患者中，还测量了肿瘤组织和正常肾实质中的超氧化物歧化酶、过氧化氢酶和脂质过氧化水平。
与正常人和良性肿瘤患者相比，肾细胞癌患者的活性氧、一氧化氮显著增加，谷胱甘肽减少。与正常肾组织相比，肿瘤组织中的超氧化物歧化酶和脂质过氧化增加，过氧化氢酶减少。氧化应激与肾细胞癌的分级和分期相关，但在根治性切除后降低。患有转移性疾病的患者的氧化应激参数持续升高。良性肿瘤组织中的抗氧化酶水平明显高于肾细胞癌。
肾细胞癌患者的氧化应激增加，根治性切除可有效缓解。在良性肿瘤患者中，抗氧化防御机制维持正常的氧化还原状态。
