Dominguez-Steglich M, Meng G, Bettecken T, Müller C R, Schmid M
Department of Human Genetics, University of Würzburg, Federal Republic of Germany.
Genomics. 1990 Nov;8(3):536-40. doi: 10.1016/0888-7543(90)90041-r.
The dystrophin gene has been mapped to a pair of microchromosomes in Gallus domesticus. In situ hybridization using a pool of biotinylated human cDNA probes allowed detection of this huge single-copy sequence without having to employ isotopic labeling. The autosomal nature of the DMD gene in chicken is supported by molecular data from quantitative Southern blot analysis and is in sharp contrast to that in all eutherian mammals studied, where it is a characteristically X-linked locus. With previous data taken into consideration, these results should prove significant in understanding the evolution of sex chromosomes during speciation as well as highlighting the importance of avian microchromosomes.
肌营养不良蛋白基因已被定位到家鸡的一对微染色体上。使用生物素化的人类cDNA探针池进行原位杂交，无需采用同位素标记就能检测到这个巨大的单拷贝序列。来自定量Southern印迹分析的分子数据支持了鸡中DMD基因的常染色体性质，这与所有已研究的真兽类哺乳动物形成鲜明对比，在这些哺乳动物中，它是一个典型的X连锁基因座。考虑到先前的数据，这些结果对于理解物种形成过程中性染色体的进化以及凸显鸟类微染色体的重要性应该具有重要意义。
