Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Nat Methods. 2012 Sep;9(9):910-2. doi: 10.1038/nmeth.2133. Epub 2012 Aug 5.
We introduce a microfluidic system for simultaneously measuring single-cell mass and cell cycle progression over multiple generations. We use this system to obtain over 1,000 h of growth data from mouse lymphoblast and pro-B-cell lymphoid cell lines. Cell lineage analysis revealed a decrease in the growth rate variability at the G1-S phase transition, which suggests the presence of a growth rate threshold for maintaining size homeostasis.
我们介绍了一种用于同时测量单细胞质量和多个代细胞周期进程的微流控系统。我们使用该系统从小鼠淋巴母细胞和前 B 细胞淋巴样细胞系中获得了超过 1000 小时的生长数据。细胞谱系分析显示，在 G1-S 期转变时，生长速率变化减小，这表明存在维持大小平衡的生长速率阈值。
