Department of Chemistry, Pennsylvania State University-York, 1031 Edgecomb Avenue, York, PA 17403, USA.
Molecules. 2012 Aug 3;17(8):9274-82. doi: 10.3390/molecules17089274.
A solvent system consisting of ethyl acetate, ethyl alcohol and water, in the volume ratio of 4.5:4.5:1, was developed and used to extract, at room temperature, betulin from white birch bark and antioxidants from spices (rosemary, thyme, sage, and oregano) and white oak chips. In addition, under reflux conditions, trimyristin was extracted from nutmeg using the same solvent system, and eugenol from olives was extracted using a mixture of salt water and ethyl acetate. The protocol demonstrates the use of water in organic solvents to extract natural products from plants. Measurement of the free-radical scavenging activity using by 2,2-diphenyl-1-picrylhydrazyl (DPPH) indicated that the extraction of plant material using ethyl acetate, ethyl alcohol and water (4.5:4.5:1, v/v/v) was exhaustive when carried out at room temperature for 96 h.
开发并使用了一种由乙酸乙酯、乙醇和水组成的溶剂体系，其体积比为 4.5:4.5:1，用于在室温下从白桦树皮中提取桦木醇和从香料（迷迭香、百里香、鼠尾草和牛至）和白橡木屑中提取抗氧化剂。此外，在回流条件下，使用相同的溶剂体系从肉豆蔻中提取三肉豆蔻精，使用盐水和乙酸乙酯的混合物从橄榄中提取丁香酚。该方案展示了在有机溶剂中使用水从植物中提取天然产物的方法。使用 2,2-二苯基-1-苦基肼（DPPH）测量自由基清除活性表明，当在室温下进行 96 小时时，使用乙酸乙酯、乙醇和水（4.5:4.5:1，v/v/v）提取植物材料是完全的。
