Hu Xuan, Wang Fengyi, Peng Qianqian, Hu Jing, Peng Huaqiao, Li Lin, Zheng Baozhan, Du Juan, Xiao Dan
College of Chemistry, Sichuan University 29 Wangjiang Road Chengdu 610064 China
The Second Research Institute of Civil Aviation Administration of China (CAAC) Chengdu 610041 China.
RSC Adv. 2019 Apr 29;9(23):13048-13053. doi: 10.1039/c9ra01748h. eCollection 2019 Apr 25.
A highly sensitive and selective luminescent sensor for the detection of methyl parathion (MP) pesticide was described in this study. The target molecule HL was synthesized by modifying the structure of 4-hydroxybenzlidene imidazolinone (HBI) with nitrogen-containing heterocyclic 1,10-phenanthroline. In the presence of Eu, a HL-Eu complex was formed which could emit strong red fluorescence due to the removal of coordinated water molecules and an intramolecular energy transfer from HL to Eu. Addition of MP into the strongly fluorescent solution of HL-Eu induced quenching of the complex's fluorescence, and this quenching behavior occurred because of the competition coordination of MP and HL for Eu. A calibration curve was developed that related the extent of fluorescence quenching to MP concentration, making the HL-Eu system a sensitive and selective fluorescent sensor for MP. Under the experimental conditions, the detection limit for MP was down to 95 nM based on LOD = 3/. Moreover, the fluorescence assay developed here allowed the detection of MP in two different types of real samples including pond water and pear juice, and satisfactory results demonstrate that this fluorescent sensor based on HL-Eu has potential application in environment and food analysis.
本研究描述了一种用于检测甲基对硫磷（MP）农药的高灵敏度和高选择性发光传感器。目标分子HL是通过用含氮杂环1,10-菲咯啉修饰4-羟基苯亚甲基咪唑啉酮（HBI）的结构合成的。在铕（Eu）存在下，形成了HL-Eu配合物，由于配位水分子的去除以及从HL到Eu的分子内能量转移，该配合物可发出强烈的红色荧光。向HL-Eu的强荧光溶液中加入MP会导致配合物荧光猝灭，这种猝灭行为是由于MP和HL对Eu的竞争配位所致。绘制了一条校准曲线，将荧光猝灭程度与MP浓度相关联，使得HL-Eu体系成为一种用于MP的灵敏且选择性的荧光传感器。在实验条件下，基于LOD = 3σ，MP的检测限低至95 nM。此外，此处开发的荧光分析法可检测两种不同类型的实际样品中的MP，包括池塘水和梨汁，令人满意的结果表明这种基于HL-Eu的荧光传感器在环境和食品分析中具有潜在应用。
