绿茶提取物：对皮肤病原体的可能作用机制和抗菌活性。

Green tea extract: possible mechanism and antibacterial activity on skin pathogens.

机构信息

Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector 62, Noida, 201307 UP, India.

出版信息

Food Chem. 2012 Nov 15;135(2):672-5. doi: 10.1016/j.foodchem.2012.04.143. Epub 2012 May 11.

DOI:10.1016/j.foodchem.2012.04.143

Abstract

Camellia sinensis (tea) is known for its therapeutic properties (anti-inflammatory, anti-microbial, anti-tumour, anti-oxidative and anti-ageing). Although, anti-microbial properties of green tea have been studied, its role against bacterial strains related to skin infections and mechanism of action is not well understood. We focussed on exploring anti-microbial activity and the basic mechanism of aqueous green tea leaf extract on selected bacterial strains. Staphylococcus epidermidis, Micrococcus luteus, Brevibacterium linens, Pseudomonas fluorescens and Bacillus subtilis were found to be sensitive to green tea extract via disc diffusion assay (zone of inhibition ≥7 mm). Minimal inhibitory concentration (MIC) was determined via nitro blue tetrazolium (NBT) assay (0.156-0.313 mg/ml). Moreover, the aqueous extract was found to be not toxic to the Vero cell-line up to a concentration of 500 μg/ml. The effect of aqueous extract on adhesion of different bacteria to Vero cells indicated that it inhibits the adhesion at its MIC value.

摘要

茶树（茶）以其治疗特性（抗炎、抗菌、抗肿瘤、抗氧化和抗衰老）而闻名。尽管已经研究了绿茶的抗菌特性，但它对与皮肤感染相关的细菌菌株的作用及其作用机制尚不清楚。我们专注于探索水提绿茶叶提取物对选定细菌菌株的抗菌活性和基本作用机制。通过圆盘扩散试验（抑菌环≥7mm）发现表皮葡萄球菌、藤黄微球菌、产色短杆菌、荧光假单胞菌和枯草芽孢杆菌对绿茶提取物敏感。通过硝基蓝四唑（NBT）试验（0.156-0.313mg/ml）确定最小抑菌浓度（MIC）。此外，水提物在高达 500μg/ml 的浓度下对非洲绿猴肾细胞系（Vero 细胞系）也没有毒性。水提物对不同细菌黏附到 Vero 细胞的影响表明，它在其 MIC 值下抑制黏附。