Jones A W, Jorfeldt L, Hjertberg H, Jönsson K A
Department of Alcohol Toxicology, University Hospital, Linköping, Sweden.
J Forensic Sci Soc. 1990 Sep-Oct;30(5):273-83. doi: 10.1016/s0015-7368(90)73355-5.
Specimens of arterial plasma and venous whole blood were obtained at 3-10 min intervals during the post-peak phase of ethanol metabolism in healthy volunteers. The concentrations of ethanol in blood and plasma were determined by headspace gas chromatography. This method had a standard deviation of 0.28 mg/dl for whole blood and 0.26 mg/dl for plasma and the coefficients of variation were 0.43% and 0.79% respectively. The physiological variation from time-to-time, expressed as the residual standard deviation after fitting the ethanol concentration-time regression relationships, ranged from 0.43-3.7 mg/dl (0.65-16%). The time-to-time variations in concentrations of ethanol were maximum when there were problems in getting an unimpeded flow of blood through the indwelling catheters. The results do not support the existence of sporadic fluctuations or spiking in the blood alcohol concentration-time profile during the post-absorptive state. Instead, this study underscores the need to control carefully the method of sampling blood and in this way keep pre-analytical sources of variation to a minimum.
在健康志愿者乙醇代谢的峰值后阶段，每隔3 - 10分钟采集动脉血浆和静脉全血样本。采用顶空气相色谱法测定血液和血浆中的乙醇浓度。该方法对全血的标准差为0.28 mg/dl，对血浆的标准差为0.26 mg/dl，变异系数分别为0.43%和0.79%。将乙醇浓度-时间回归关系拟合后，以残差标准差表示的时间间生理变异范围为0.43 - 3.7 mg/dl（0.65 - 16%）。当通过留置导管获得通畅血流存在问题时，乙醇浓度的时间间变化最大。结果不支持在吸收后状态下血液酒精浓度-时间曲线存在散发性波动或峰值。相反，本研究强调需要仔细控制采血方法，从而将分析前的变异来源降至最低。
