Brien Holden Vision Institute, University of New South Wales, Sydney, Australia.
Eye Contact Lens. 2012 Nov;38(6):394-9. doi: 10.1097/ICL.0b013e318261aa13.
To examine the effectiveness of heating contact lens cases after disinfection on reducing microbial contamination.
One strain each of Pseudomonas aeruginosa (071) and Staphylococcus aureus (31) were used to set up robust biofilms in polypropylene contact lens cases. The effect of dilutions (from 1:10 to 1:1000) of trypticase soy broth (TSB) in phosphate-buffered saline and incubation time (24 to 48h) on the ability of strains to from biofilms with high levels of bacteria were first examined. Then the effect of increasing the temperature of incubation (from 14°C to 60°C) of biofilms during drying was examined. In the final set of experiments, biofilms of strains were subjected to heating in a warming device set to deliver 60°C for 3 hours, and the effect of this temperature after disinfection with a multipurpose disinfecting solution (MPDS; containing polyquat and Aldox) was examined by culturing the number of viable bacterial cells remaining.
A dilution of 1:100 TSB for S. aureus 31 and 1:1000 TSB for P. aeruginosa 071 together with an incubation time of 24 hours gave high numbers of viable cells of these 2 strains adhered to the contact lens cases. Having established the biofilms of bacteria, heating these to 60°C for 3 hours resulted in significant reductions in the number of viable cells that could be cultured (1 log reduction for S. aureus 31, P=0.0003; 3.5 log reduction for P. aeruginosa 071, P=0.002). Exposing the biofilms of cells to a disinfection cycle (6h at ambient temperature) in the presence of the MPDS and air drying at ambient temperature resulted in 2441±1237 colony-forming units/lens well for S. aureus 31 and 7401±4374 colony-forming units/lens well for P. aeruginosa 071. Increasing the drying temperature to 60°C resulted in zero viable cells (i.e., ≥4log reduction) for either bacterial type.
Using a warming device for contact lens cases after a disinfection cycle with an MPDS during drying for 3 hours results in substantial kill of biofilms of P. aeruginosa and S. aureus that have been formed in the wells of the cases.
研究消毒后加热隐形眼镜盒对减少微生物污染的效果。
使用铜绿假单胞菌（071）和金黄色葡萄球菌（31）各一株在聚丙烯隐形眼镜盒中建立稳定的生物膜。首先检查胰蛋白酶大豆肉汤（TSB）稀释度（从 1:10 至 1:1000）和磷酸盐缓冲盐水孵育时间（24 至 48 小时）对高浓度细菌形成生物膜能力的影响。然后，检查在干燥过程中增加生物膜孵育温度（从 14°C 至 60°C）的效果。在最后一组实验中，将菌株的生物膜置于加热装置中，设定 60°C 加热 3 小时，然后用多用途消毒剂（MPDS；含有聚季铵盐和 Aldox）消毒后，检查该温度对存活细菌数量的影响。
金黄色葡萄球菌 31 用 1:100 TSB 稀释，铜绿假单胞菌 071 用 1:1000 TSB 稀释，孵育时间为 24 小时，可获得大量附着在隐形眼镜盒上的这两种菌株的活菌细胞。在建立了细菌生物膜后，将其加热至 60°C 3 小时可显著减少可培养的活菌细胞数量（金黄色葡萄球菌 31 减少 1 对数，P=0.0003；铜绿假单胞菌 071 减少 3.5 对数，P=0.002）。将生物膜暴露于含有 MPDS 的环境温度下的消毒循环（6 小时）和在环境温度下空气干燥，可导致金黄色葡萄球菌 31 每个镜片盒孔的菌落形成单位数为 2441±1237，铜绿假单胞菌 071 每个镜片盒孔的菌落形成单位数为 7401±4374。将干燥温度提高到 60°C 可导致两种细菌类型均无活菌（即减少≥4 对数）。
使用 MPDS 进行消毒循环后，在干燥 3 小时期间使用加热装置对隐形眼镜盒进行加热，可有效杀灭已在盒孔中形成的铜绿假单胞菌和金黄色葡萄球菌生物膜。
