Zhang H B, Wei S G, Yu B, Li L, Lai J H
Key Laboratory of the National Ministry of Health for Forensic Sciences, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
Genet Mol Res. 2012 Aug 16;11(3):2534-8. doi: 10.4238/2012.July.10.8.
A large number of microsatellite genetic markers have been identified in the human leukocyte antigen (HLA) region. We investigated genetic polymorphism of the nine short tandem repeat (STR) loci (D6S276, MOGCA, D6S265, MIB, D6S273, G51152, TAP1CA, RING3CA, and D6S291) in the HLA region in the Shaanxi Han population. Using a fluorescence-labeled multiplex-PCR STR typing method, 6-13 alleles were detected in these nine STR loci in 150 unrelated Han Chinese from the region of Shaanxi, China. The distributions of the genotypes at these nine loci were in Hardy-Weinberg equilibrium. We conclude that these nine STR loci have a high level of genetic polymorphism; they would be useful for population genetic studies, pre-transplantation HLA typing, forensic and paternity testing, etc.
人类白细胞抗原（HLA）区域已鉴定出大量微卫星遗传标记。我们研究了陕西汉族人群HLA区域中9个短串联重复序列（STR）位点（D6S276、MOGCA、D6S265、MIB、D6S273、G51152、TAP1CA、RING3CA和D6S291）的基因多态性。采用荧光标记多重PCR STR分型方法，在中国陕西地区150名无亲缘关系的汉族个体中，这9个STR位点检测到6 - 13个等位基因。这9个位点的基因型分布符合哈迪 - 温伯格平衡。我们得出结论，这9个STR位点具有高度的基因多态性；它们将有助于群体遗传学研究、移植前HLA分型、法医鉴定和亲子鉴定等。
